Literature DB >> 6699153

Detection of Clostridium difficile toxin with McCoy cell monolayers and cell suspensions and comparison with HeLa cell assay.

A C Maniar, H Chubb, T J Louie, T W Williams, W Forsyth, J C Wilt.   

Abstract

McCoy cell monolayers were compared with HeLa cell monolayers for the detection of Clostridium difficile toxin in 301 stool samples. Tests were positive (greater than or equal to 1/100 dilution) in 83 and 81 specimens tested with McCoy and HeLa cell monolayers, respectively. McCoy cell suspensions were compared with HeLa cell monolayers in 532 stool filtrates. Overall, 90 positive specimens were within one dilution and 432 filtrates were negative with either test, giving a correlation coefficient of r = 0.98. McCoy cell monolayers or suspensions may be a satisfactory substitute for the detection of C. difficile toxin in clinical specimens.

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Year:  1984        PMID： 6699153      PMCID： PMC271042          DOI： 10.1128/jcm.19.2.294-295.1984

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  CYTOTOXIC TEST FOR DEMONSTRATION OF MOUSE ANTIBODY.

Authors:  E A BOYSE; L J OLD; I CHOUROULINKOV
Journal:  Methods Med Res       Date:  1964

2.  Cytotoxicity assay in antibiotic-associated colitis.

Authors:  T W Chang; M Lauermann; J G Bartlett
Journal:  J Infect Dis       Date:  1979-11       Impact factor: 5.226

3.  Effects of Clostridium difficile toxin on tissue-cultured cells.

Authors:  S T Donta; S J Shaffer
Journal:  J Infect Dis       Date:  1980-02       Impact factor: 5.226

4.  Selective and differential medium for isolation of Clostridium difficile.

Authors:  W L George; V L Sutter; D Citron; S M Finegold
Journal:  J Clin Microbiol       Date:  1979-02       Impact factor: 5.948

5.  Interaction of cytopathogenic toxin from Clostridium difficile with cells in tissue culture.

Authors:  M Thelestam; M Brönnegård
Journal:  Scand J Infect Dis Suppl       Date:  1980
  5 in total
  5 in total

1.  Detection of verotoxin in stool specimens.

Authors:  A C Maniar; T Williams; C M Anand; G W Hammond
Journal:  J Clin Microbiol       Date:  1990-01       Impact factor: 5.948

2.  Predominant Staphylococcus aureus isolated from antibiotic-associated diarrhea is clinically relevant and produces enterotoxin A and the bicomponent toxin LukE-lukD.

Authors:  A Gravet; M Rondeau; C Harf-Monteil; F Grunenberger; H Monteil; J M Scheftel; G Prévost
Journal:  J Clin Microbiol       Date:  1999-12       Impact factor: 5.948

3.  Effects of Clostridium difficile toxin B on human monocytes and macrophages: possible relationship with cytoskeletal rearrangement.

Authors:  J C Siffert; O Baldacini; J G Kuhry; D Wachsmann; S Benabdelmoumene; A Faradji; H Monteil; P Poindron
Journal:  Infect Immun       Date:  1993-03       Impact factor: 3.441

4.  Detection of Clostridium difficile toxin in various tissue culture monolayers.

Authors:  A C Maniar; T W Williams; G W Hammond
Journal:  J Clin Microbiol       Date:  1987-10       Impact factor: 5.948

Review 5.  Laboratory diagnosis of Clostridium difficile-associated diarrhoea.

Authors:  R A Bowman; T V Riley
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1988-08       Impact factor: 3.267
  5 in total

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