Female behavior is affected by male ultrasonic vocalizations in house mice.

The effects of male-mouse ultrasonic vocalizations on female preference behavior were investigated using Swiss-Webster mice. Intact females spent more time with an intact tethered male than with a devocalized tethered male during 3-min preference tests. Female preference behavior was similar during estrus and diestrus; but, a preference for the vocalizing male was absent after ovariectomy, and returned following replacement treatment with ovarian hormones. Similar effects on female behavior were obtained using synthetic 70 kHz ultrasounds presented behind one of two devocalized tethered males during preference tests. The results indicate that ultrasounds maintain the female close to the vocalizing male and may facilitate copulation.