Literature DB >> 6656994

Anti-human choline acetyltransferase fragments antigen binding (FAB)-sepharose chromatography for enzyme purification.

J H Peng, P L McGeer, E G McGeer.   

Abstract

Fragments Antigen Binding (FAB)-Sepharose immunoaffinity chromatography was successfully applied to the purification of human choline acetyltransferase. The mild elution conditions made it possible to obtain the enzyme in its full catalytic state. The method should be generally applicable to enzyme purification.

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Year:  1983        PMID： 6656994     DOI： 10.1007/BF00965003

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  16 in total

1.  The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens.

Authors:  R Standring; W R McMaster; C A Sunderland; A F Williams
Journal:  Eur J Immunol       Date:  1978-12       Impact factor: 5.532

2.  A simplified method for cyanogen bromide activation of agarose for affinity chromatography.

Authors:  S C March; I Parikh; P Cuatrecasas
Journal:  Anal Biochem       Date:  1974-07       Impact factor: 3.365

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Analysis of sperm antigens by sodium dodecyl sulfate gel/protein blot radioimmunobinding method.

Authors:  C Y Lee; Y S Huang; P C Hu; V Gomel; A C Menge
Journal:  Anal Biochem       Date:  1982-06       Impact factor: 3.365

5.  Immunoaffinity chromatography of proteins.

Authors:  D M Livingston
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

6.  The central cholinergic system studied by choline acetyltransferase immunohistochemistry in the cat.

Authors:  H Kimura; P L McGeer; J H Peng; E G McGeer
Journal:  J Comp Neurol       Date:  1981-08-01       Impact factor: 3.215

7.  Immunochemical studies of bovine and human choline-O-acetyltransferase using monoclonal antibodies.

Authors:  A I Levey; D B Rye; B H Wainer
Journal:  J Neurochem       Date:  1982-12       Impact factor: 5.372

8.  A fluorometric assay for choline acetyltransferase and its use in the purification of the enzyme from human placenta.

Authors:  L B Hersh; B Coe; L Casey
Journal:  J Neurochem       Date:  1978-05       Impact factor: 5.372

9.  Anti-choline acetyltransferase fragments antigen binding (Fab) for immunohistochemistry.

Authors:  J H Peng; H Kimura; P L McGeer; E G McGeer
Journal:  Neurosci Lett       Date:  1981-02-06       Impact factor: 3.046

10.  Choline acetyltransferase-containing neurons in rodent brain demonstrated by immunohistochemistry.

Authors:  H Kimura; P L McGeer; F Peng; E G McGeer
Journal:  Science       Date:  1980-05-30       Impact factor: 47.728
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  4 in total

Review 1.  Molecular biology and neurobiology of choline acetyltransferase.

Authors:  P M Salvaterra
Journal:  Mol Neurobiol       Date:  1987       Impact factor: 5.590

2.  Studies on detergent released choline acetyltransferase from membrane fractions of rat and human brain.

Authors:  G Bruce; L B Hersh
Journal:  Neurochem Res       Date:  1987-12       Impact factor: 3.996

3.  Choline Acetyltransferase Mutations Causing Congenital Myasthenic Syndrome: Molecular Findings and Genotype-Phenotype Correlations.

Authors:  Juan Arredondo; Marian Lara; Sídney M Gospe; Claudio G Mazia; Maria Vaccarezza; Marcela Garcia-Erro; Constance M Bowe; Celia H Chang; Michelle M Mezei; Ricardo A Maselli
Journal:  Hum Mutat       Date:  2015-07-24       Impact factor: 4.878

4.  Membrane-bound choline acetyltransferase from human brain: purification and properties.

Authors:  J H Peng; P L McGeer; E G McGeer
Journal:  Neurochem Res       Date:  1986-07       Impact factor: 3.996
  4 in total

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