Literature DB >> 2450285

Studies on detergent released choline acetyltransferase from membrane fractions of rat and human brain.

G Bruce1, L B Hersh.   

Abstract

The relationship between soluble and membrane choline acetyltransferase (ChAT) was studied. Differential solubilization of rat and human brain yielded ChAT in the soluble and membrane fractions. The addition of 1% Triton X-100 to membrane fractions resulted in a release of ChAT. A comparable release of lactate dehydrogenase was also observed. The Triton released ChAT and soluble ChAT from rat and human brain were efficiently purified by immuno-affinity chromatography. A single molecular weight of 68,000 was observed for both forms of rat and human brain ChAT. Epitope maps produced from both forms of human brain ChAT were identical. It is concluded that Triton released ChAT is identical to soluble ChAT and simply represents occluded soluble ChAT.

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Year:  1987        PMID： 2450285     DOI： 10.1007/bf00971705

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  22 in total

1.  The intracellular distribution of glycolytic and other enzymes in rat-brain homogenates and mitochondrial preparations.

Authors:  M K JOHNSON
Journal:  Biochem J       Date:  1960-12       Impact factor: 3.857

2.  A rapid radiochemical method for the determination of choline acetyltransferase.

Authors:  F Fonnum
Journal:  J Neurochem       Date:  1975-02       Impact factor: 5.372

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Choline acetyltransferase binding to and release from membranes.

Authors:  F Fonnum
Journal:  Biochem J       Date:  1968-09       Impact factor: 3.857

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  The separation of synaptic vesicles from nerve-ending particles ('synaptosomes').

Authors:  V P Whittaker; I A Michaelson; R J Kirkland
Journal:  Biochem J       Date:  1964-02       Impact factor: 3.857

Review 7.  Choline acetyltransferase: purification and immunohistochemical localization.

Authors:  P L McGeer; E G McGeer; J H Peng
Journal:  Life Sci       Date:  1984-06-11       Impact factor: 5.037

8.  Immunoaffinity purification of human choline acetyltransferase: comparison of the brain and placental enzymes.

Authors:  G Bruce; B H Wainer; L B Hersh
Journal:  J Neurochem       Date:  1985-08       Impact factor: 5.372

9.  Is choline acetyltransferase present in synaptic vesicles?

Authors:  F Fonnum
Journal:  Biochem Pharmacol       Date:  1966-10       Impact factor: 5.858

10.  Membrane-bound choline acetyltransferase from human brain: purification and properties.

Authors:  J H Peng; P L McGeer; E G McGeer
Journal:  Neurochem Res       Date:  1986-07       Impact factor: 3.996
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  2 in total

1.  Spinal cord from body donors is suitable for multicolor immunofluorescence.

Authors:  Lukas F Reissig; Genova Carrero-Rojas; Udo Maierhofer; Atieh Seyedian Moghaddam; Andreas Hainfellner; Bernhard Gesslbauer; Thomas Haider; Johannes Streicher; Oskar C Aszmann; Angel M Pastor; Wolfgang J Weninger; Roland Blumer
Journal:  Histochem Cell Biol       Date:  2022-10-06       Impact factor: 2.531

2.  Choline acetyltransferase-like activity bound to neuronal plasma membranes.

Authors:  R Massarelli; B Ferret; G Sorrentino; H Hattori; J N Kanfer
Journal:  Neurochem Res       Date:  1988-12       Impact factor: 3.996
  2 in total

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