Literature DB >> 7012696

Anti-choline acetyltransferase fragments antigen binding (Fab) for immunohistochemistry.

J H Peng, H Kimura, P L McGeer, E G McGeer.   

Abstract

The preparation of fragments antigen binding (Fab) from serum of rabbits immunized against human brain choline acetyltransferase (CAT) is described. Ant-CAT Fab fragments were less potent than anti-CAT immunoglobulin (IgG) in inhibiting mammalian CAT activity and did not precipitate CAT activity. They proved, however, far superior to anti-CAT IgG for the immunohistochemical staining of CAT in mammalian neurons.

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Year:  1981        PMID: 7012696     DOI: 10.1016/0304-3940(81)90217-2

Source DB:  PubMed          Journal:  Neurosci Lett        ISSN: 0304-3940            Impact factor:   3.046


  2 in total

Review 1.  Molecular biology and neurobiology of choline acetyltransferase.

Authors:  P M Salvaterra
Journal:  Mol Neurobiol       Date:  1987       Impact factor: 5.590

2.  Anti-human choline acetyltransferase fragments antigen binding (FAB)-sepharose chromatography for enzyme purification.

Authors:  J H Peng; P L McGeer; E G McGeer
Journal:  Neurochem Res       Date:  1983-11       Impact factor: 3.996

  2 in total

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