Gradient optimization principles in reversed-phase high-performance liquid chromatography and the separation of influenza virus components.

Reversed-phase high-performance liquid chromatography is used to separate the major proteins of A/Bangkok/1/79 X 73 influenza virus. The purity of the isolated proteins, their yield and reactivity with monoclonal antibodies are discussed. The virus, in turn, is used as a probe to examine some theoretical gradient principles relating resolution, retention time and gradient time. Empirical compliance with these principles is generally shown.