Literature DB >> 2985795

Isolation by high-performance liquid chromatography and partial characterization of a 57,000-dalton herpes simplex virus type 1 polypeptide.

S Welling-Wester, T Popken-Boer, J B Wilterdink, J van Beeumen, G W Welling.   

Abstract

A Nonidet P-40 extract of HSV-1-purified virions was fractionated by reversed-phase high-performance liquid chromatography (RP-HPLC). The first peak fraction eluted at 25% organic solvent. Polyacrylamide gel electrophoresis showed that it contained a 57,000-dalton polypeptide. The polypeptide was characterized by determination of the amino acid composition and the N-terminal amino acid sequence. Adsorption of the detergent extract before RP-HPLC showed that the polypeptide reacted with monoclonal antibodies LP1 directed against herpes simplex virus polypeptide VP-16.

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Year:  1985        PMID： 2985795      PMCID： PMC254793     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  49 in total

1.  Physical mapping of herpes simplex virus-induced polypeptides.

Authors:  H S Marsden; N D Stow; V G Preston; M C Timbury; N M Wilkie
Journal:  J Virol       Date:  1978-11       Impact factor: 5.103

2.  Polypeptide synthesized in herpes simplex virus type 2-infected HEp-2 cells.

Authors:  K L Powell; R J Courtney
Journal:  Virology       Date:  1975-07       Impact factor: 3.616

3.  Membrane proteins specified by herpes simplex viruses. I. Identification of four glycoprotein precursors and their products in type 1-infected cells.

Authors:  P G Spear
Journal:  J Virol       Date:  1976-03       Impact factor: 5.103

4.  Revolution in liquid chromatography.

Authors:  J N Done; G J Kennedy; J H Knox
Journal:  Nature       Date:  1972-05-12       Impact factor: 49.962

5.  Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and nonstructural herpes virus polypeptides in the infected cell.

Authors:  R W Honess; B Roizman
Journal:  J Virol       Date:  1973-12       Impact factor: 5.103

6.  Proteins specified by herpes simplex virus. Staining and radiolabeling properties of B capsid and virion proteins in polyacrylamide gels.

Authors:  W Gibson; B Roizman
Journal:  J Virol       Date:  1974-01       Impact factor: 5.103

7.  Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion.

Authors:  P G Spear; B Roizman
Journal:  J Virol       Date:  1972-01       Impact factor: 5.103

8.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

9.  Comparison of the virion proteins specified by herpes simplex virus types 1 and 2.

Authors:  E N Cassai; M Sarmiento; P G Spear
Journal:  J Virol       Date:  1975-11       Impact factor: 5.103

10.  Proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains.

Authors:  J W Heine; R W Honess; E Cassai; B Roizman
Journal:  J Virol       Date:  1974-09       Impact factor: 5.103
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  1 in total

1.  Nucleotide sequence and predicted amino acid sequence of a protein encoded in a small herpes simplex virus DNA fragment capable of trans-inducing alpha genes.

Authors:  P E Pellett; J L McKnight; F J Jenkins; B Roizman
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205
  1 in total

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