| Literature DB >> 6630149 |
M N Vijayakumar, D A Morrison.
Abstract
Uptake of DNA and genetic recombination proceeded normally in competent Streptococcus pneumoniae despite inhibition of DNA replication by 6-(p-hydroxyphenylazo)-uracil. Immediately after a brief uptake period, 68% of donor DNA label was in eclipse complex form, and 22% was in low-molecular-weight products; by the completion of integration at 10 min, 23% was integrated into the chromosome, and the rest was lost from the cell. Throughout the process, less than 1% was found as free single strands. The DNA in eclipse complex is therefore an intermediate in the integration process.Entities:
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Year: 1983 PMID： 6630149 PMCID： PMC217878 DOI： 10.1128/jb.156.2.644-648.1983
Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490