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Literature DB >> 21166

Transformation in pneumococcus: existence and properties of a complex involving donor deoxyribonucleate single strands in eclipse.

Abstract

Donor deoxyribonucleic acid (DNA) single strands exist in a complex during the eclipse phase in pneumococcal transformation. This eclipse complex exhibited specific physical properties distinct from those of both pure DNA single strands and native DNA. These included a lower affinity for diethylaminoethyl-cellulose and hydroxylapatite than that of single-strand DNA, faster sedimentation than the DNA chains that it contains, and a buoyant density in Cs2SO4 lower than that of native DNA. The complex was dissociated by treatments with sodium dodecyl sulfate, NaOH, guanidine-hydrochloride, chloroform, and proteinase K but was insensitive to ribonuclease.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1977 PMID： 21166 PMCID： PMC221898 DOI： 10.1128/jb.132.2.576-583.1977

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

25 in total

1. ON THE MECHANISM OF DEOXYRIBONUCLEATE INTEGRATION IN PNEUMOCOCCAL TRANSFORMATION.

Authors: M S FOX; M K ALLEN
Journal: Proc Natl Acad Sci U S A Date: 1964-08 Impact factor: 11.205

2. Evidence for message reading from a unique strand of pneumococcal DNA.

Authors: W R GUILD; M ROBINSON
Journal: Proc Natl Acad Sci U S A Date: 1963-07 Impact factor: 11.205

3. Transformation by denatured deoxyribonucleic acid.

Authors: W R GUILD
Journal: Proc Natl Acad Sci U S A Date: 1961-10-15 Impact factor: 11.205

4. [The status of the transforming DNA during the 1st phases of bacterial transformation].

Authors: H EPHRUSSI-TAYLOR
Journal: C R Seances Soc Biol Fil Date: 1960

5. Fate of transforming deoxyribonucleate following fixation by transformable bacteria.

Authors: M S FOX; R D HOTCHKISS
Journal: Nature Date: 1960-09-17 Impact factor: 49.962

6. Genetic transformation. I. Cellular incorporation of DNA accompanying transformation in Pneumococcus.

Authors: L S LERMAN; L J TOLMACH
Journal: Biochim Biophys Acta Date: 1957-10

7. Protein chromatography on calcium phosphate columns.

Authors: S HJERTEN; O LEVIN; A TISELIUS
Journal: Arch Biochem Biophys Date: 1956-11 Impact factor: 4.013

8. Molecular fate of DNA in genetic transformation of Pneumococcus.

Authors: S LACKS
Journal: J Mol Biol Date: 1962-07 Impact factor: 5.469

9. Fate of transforming DNA following uptake by competent Bacillus subtilis. Formation and properties of products isolated from transformed cells which are derived entirely from donor DNA.

Authors: D Dubnau; C Cirigliano
Journal: J Mol Biol Date: 1972-02-28 Impact factor: 5.469

10. Fate of transforming deoxyribonucleate in Bacillus subtilis.

Authors: M Piechowska; M S Fox
Journal: J Bacteriol Date: 1971-11 Impact factor: 3.490

13 in total

1. Genetic transformation in Streptococcus pneumoniae: molecular cloning and characterization of recP, a gene required for genetic recombination.

Authors: D K Rhee; D A Morrison
Journal: J Bacteriol Date: 1988-02 Impact factor: 3.490

9. Isolation and characterization of three new classes of transformation-deficient mutants of Streptococcus pneumoniae that are defective in DNA transport and genetic recombination.

Authors: D A Morrison; S A Lacks; W R Guild; J M Hageman
Journal: J Bacteriol Date: 1983-10 Impact factor: 3.490

10. Fate of homospecific transforming DNA bound to Streptococcus sanguis.

Authors: J L Raina; A W Ravin
Journal: J Bacteriol Date: 1978-03 Impact factor: 3.490

Transformation in pneumococcus: existence and properties of a complex involving donor deoxyribonucleate single strands in eclipse.

1. ON THE MECHANISM OF DEOXYRIBONUCLEATE INTEGRATION IN PNEUMOCOCCAL TRANSFORMATION.

2. Evidence for message reading from a unique strand of pneumococcal DNA.

3. Transformation by denatured deoxyribonucleic acid.

4. [The status of the transforming DNA during the 1st phases of bacterial transformation].

5. Fate of transforming deoxyribonucleate following fixation by transformable bacteria.

6. Genetic transformation. I. Cellular incorporation of DNA accompanying transformation in Pneumococcus.

7. Protein chromatography on calcium phosphate columns.

8. Molecular fate of DNA in genetic transformation of Pneumococcus.

9. Fate of transforming DNA following uptake by competent Bacillus subtilis. Formation and properties of products isolated from transformed cells which are derived entirely from donor DNA.

10. Fate of transforming deoxyribonucleate in Bacillus subtilis.

1. Genetic transformation in Streptococcus pneumoniae: molecular cloning and characterization of recP, a gene required for genetic recombination.

2. Identification of the major protein component of the pneumococcal eclipse complex.

3. Transformation in pneumococcus: protein content of eclipse complex.

4. Localization of competence-induced proteins in Streptococcus pneumoniae.

5. The rec locus, a competence-induced operon in Streptococcus pneumoniae.

6. Fate of DNA in eclipse complex during genetic transformation in Streptococcus pneumoniae.

Review 7. Translocation of DNA across bacterial membranes.

Review 8. Transformation in Haemophilus: a problem in membrane biology.

9. Isolation and characterization of three new classes of transformation-deficient mutants of Streptococcus pneumoniae that are defective in DNA transport and genetic recombination.

10. Fate of homospecific transforming DNA bound to Streptococcus sanguis.