Literature DB >> 6605975

Enzyme-linked immunosorbent assay for detection of capsular antibodies against Haemophilus influenzae type b: comparison with radioimmunoassay.

S L Kaplan, E O Mason, G Johnson, R A Broughton, D Hurley, J C Parke.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) is described for detection of anti-capsular antibodies against Haemophilus influenzae type b. Polyribosephosphate was covalently bonded to poly-L-lysine before adsorption to microtiter plates. ELISA immunoglobulin G and immunoglobulin M anti-polyribosephosphate antibody titers were comparable to total anti-polyribosephosphate antibody concentration determined by radioimmunoassay. The ELISA technique will be useful for further investigations of host response to infections due to H. influenzae type b but is not intended to be used as a serological method for documenting H. influenzae type b infections.

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Year:  1983        PMID: 6605975      PMCID: PMC272867          DOI: 10.1128/jcm.18.5.1201-1204.1983

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  12 in total

1.  Preparation of iodine-131 labelled human growth hormone of high specific activity.

Authors:  W M HUNTER; F C GREENWOOD
Journal:  Nature       Date:  1962-05-05       Impact factor: 49.962

2.  Quantitative measurement of "natural" and immunization-induced Haemophilus influenzae type b capsular polysaccharide antibodies.

Authors:  J B Robbins; J C Parke; R Schneerson; J K Whisnant
Journal:  Pediatr Res       Date:  1973-03       Impact factor: 3.756

3.  Indirect enzyme-linked immunosorbent assay for the quantitation of the type-specific antigen of Haemophilus influenzae b: a preliminary report.

Authors:  J Pepple; E R Moxon; R H Yolken
Journal:  J Pediatr       Date:  1980-08       Impact factor: 4.406

4.  Enzyme-linked immunosorbent assay for detection of antibodies against Streptococcus pneumoniae capsular polysaccharides.

Authors:  H Russell; L R Edwards; E W Wortham; R R Facklam
Journal:  J Clin Microbiol       Date:  1980-02       Impact factor: 5.948

5.  Immunoglobulin G and M antibodies to pneumococcal polysaccharides detected by enzyme-linked immunosorbent assay.

Authors:  D J Barrett; A J Ammann; S Stenmark; D W Wara
Journal:  Infect Immun       Date:  1980-02       Impact factor: 3.441

6.  ELISA methodology for polysaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes.

Authors:  B M Gray
Journal:  J Immunol Methods       Date:  1979       Impact factor: 2.303

7.  Detection of respiratory syncytial virus in nasal secretions from infants by enzyme-linked immunosorbent assay.

Authors:  R K Chao; M Fishaut; J D Schwartzman; K McIntosh
Journal:  J Infect Dis       Date:  1979-04       Impact factor: 5.226

8.  Breast milk antibody to the capsular polysaccharide of Haemophilus influenzae type b.

Authors:  M E Pichichero; A E Sommerfelt; M C Steinhoff; R A Insel
Journal:  J Infect Dis       Date:  1980-11       Impact factor: 5.226

9.  Quantitative determination of the human immune response to immunization with meningococcal vaccines.

Authors:  E C Gotschlich; M Rey; R Triau; K J Sparks
Journal:  J Clin Invest       Date:  1972-01       Impact factor: 14.808

10.  Quantitation of antibodies to Haemophilus influenzae type b in humans by enzyme-linked immunosorbent assay.

Authors:  T Dahlberg
Journal:  J Clin Microbiol       Date:  1981-06       Impact factor: 5.948

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  5 in total

1.  Development of serum antibodies of the immunoglobulin G class and subclasses against the capsular polysaccharide of Haemophilus influenzae type b in children and adults with invasive infections.

Authors:  B A Claesson; T Lagergård; B Trollfors
Journal:  J Clin Microbiol       Date:  1988-12       Impact factor: 5.948

2.  Generation of chemotactic activity in serum by Haemophilus influenzae type b.

Authors:  M F Tosi; S L Kaplan; E O Mason; G J Buffone; D C Anderson
Journal:  Infect Immun       Date:  1984-02       Impact factor: 3.441

3.  Serum antibody response to capsular polysaccharide, outer membrane, and lipooligosaccharide in children with invasive Haemophilus influenzae type b infections.

Authors:  B A Claesson; T Lagergård; B Trollfors; L Gothefors; U Jodal
Journal:  J Clin Microbiol       Date:  1987-12       Impact factor: 5.948

4.  Comparison between radioimmunoassay and direct and indirect enzyme-linked immunosorbent assays for determination of antibodies against Haemophilus influenzae type b capsular polysaccharide.

Authors:  T Lagergård; B Trollfors; B A Claesson; R Schneerson; J B Robbins
Journal:  J Clin Microbiol       Date:  1988-12       Impact factor: 5.948

5.  Development and Optimization of a Homemade ELISA Kit for Detection of Antibodies Against Haemophilus influenzae Type b.

Authors:  Seyed Fazlolah Mousavi; Sara Fatemi; Seyed Davar Siadat; Seyed Mohsen Zahraei; Elnaz Nikanpour; Mohamad Ali Malekan; Ali Reza Khabiri; Ali Reza Janani
Journal:  Jundishapur J Microbiol       Date:  2016-02-17       Impact factor: 0.747

  5 in total

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