| Literature DB >> 6605975 |
S L Kaplan, E O Mason, G Johnson, R A Broughton, D Hurley, J C Parke.
Abstract
An enzyme-linked immunosorbent assay (ELISA) is described for detection of anti-capsular antibodies against Haemophilus influenzae type b. Polyribosephosphate was covalently bonded to poly-L-lysine before adsorption to microtiter plates. ELISA immunoglobulin G and immunoglobulin M anti-polyribosephosphate antibody titers were comparable to total anti-polyribosephosphate antibody concentration determined by radioimmunoassay. The ELISA technique will be useful for further investigations of host response to infections due to H. influenzae type b but is not intended to be used as a serological method for documenting H. influenzae type b infections.Entities:
Mesh:
Substances:
Year: 1983 PMID: 6605975 PMCID: PMC272867 DOI: 10.1128/jcm.18.5.1201-1204.1983
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948