| Literature DB >> 38284 |
Abstract
A method is described which permits the adaption of ELISA techniques for measurement of antibody against bacterial polysaccharides. First, the polysaccharides antigen is covalently bound to poly-L-lysine, using cyanuric chloride as the coupling agent. The poly-L-lysine then adsorbs to the walls of plastic tubes, thus immobilizing the polysaccharide coupled to the poly-L-lysine. The method is simple, rapid, and utilizes small amounts of polysaccharide antigen.Entities:
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Year: 1979 PMID： 38284 DOI： 10.1016/0022-1759(79)90340-5
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303