Literature DB >> 986365

Defined nutrient medium for the in vitro maintenance of Xenopus laevis oocytes.

J J Eppig, J N Dumont.   

Abstract

A procedure is described for the isolation and culture of large numbers of follicle cell-free Xenopus laevis oocytes in all stages of development. The isolation procedure involves the incubation of pieces of ovary in a calcium-free solution OR2 containing 0.2% collagenase. A defined nutrient medium for the maintenace of the oocytes in vitro is presented. It is shown that this medium, referred to as DNOM, can maintain certain morpological and functional characteristics of oocytes for periods up to 3 weeks.

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Year:  1976        PMID： 986365     DOI： 10.1007/bf02806021

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  15 in total

1.  Amino acid metabolism in mammalian cell cultures.

Authors:  H EAGLE
Journal:  Science       Date:  1959-08-21       Impact factor: 47.728

2.  Ribosomal protein synthesis in Xenopus laevis oocytes.

Authors:  R L Hallberg; D C Smith
Journal:  Dev Biol       Date:  1975-01       Impact factor: 3.582

3.  Amino acid pools in developing oocytes of Xenopus laevis.

Authors:  J J Eppig; J N Dumont
Journal:  Dev Biol       Date:  1972-07       Impact factor: 3.582

4.  Synthesis of RNA in oocytes of Xenopus laevis during culture in vitro.

Authors:  A Colman
Journal:  J Embryol Exp Morphol       Date:  1974-10

5.  Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.

Authors:  R A Wallace; D W Jared; J N Dumont; M W Sega
Journal:  J Exp Zool       Date:  1973-06

6.  Protein uptake in vitro by amphibian oocytes.

Authors:  D W Jared; R A Wallace
Journal:  Exp Cell Res       Date:  1969-10       Impact factor: 3.905

7.  A simple colorimetric method for determination of protein.

Authors:  S Bramhall; N Noack; M Wu; J R Loewenberg
Journal:  Anal Biochem       Date:  1969-10-01       Impact factor: 3.365

8.  Protein incorporation by isolated amphibian oocytes. I. Preliminary studies.

Authors:  R A Wallace; D W Jared; B L Nelson
Journal:  J Exp Zool       Date:  1970-11

9.  Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes.

Authors:  J B Gurdon
Journal:  J Embryol Exp Morphol       Date:  1968-11

10.  [Biochemical studies on oogenesis. I. RNA synthesis and accumulation during oogenesis of the South African toad Xenopus laevis].

Authors:  M Mairy; H Denis
Journal:  Dev Biol       Date:  1971-02       Impact factor: 3.582
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  15 in total

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Journal:  Proc Natl Acad Sci U S A       Date:  2013-09-05       Impact factor: 11.205

5.  Neural-inducing activity of nuclei and nuclear fractions from Xenopus laevis embryos.

Authors:  Günther Bretzel; Hildegard Tiedemann
Journal:  Rouxs Arch Dev Biol       Date:  1986-02

6.  Tissue specific nuclear antigens in the germinal vesicle ofXenopus laevis oocytes.

Authors:  Christine Dreyer; Helen Singer; Peter Hausen; Ursula Müller; Elisabeth Siegel
Journal:  Wilehm Roux Arch Dev Biol       Date:  1981-07

7.  Calmodulin dependence of transferrin receptor recycling in rat reticulocytes.

Authors:  J A Grasso; M Bruno; A A Yates; L T Wei; P M Epstein
Journal:  Biochem J       Date:  1990-02-15       Impact factor: 3.857

8.  Long-term growth and differentiation of Xenopus oocytes in a defined medium.

Authors:  R A Wallace; Z Misulovin
Journal:  Proc Natl Acad Sci U S A       Date:  1978-11       Impact factor: 11.205

9.  Insulin induction of Xenopus laevis oocyte maturation is inhibited by monoclonal antibody against p21 ras proteins.

Authors:  A K Deshpande; H F Kung
Journal:  Mol Cell Biol       Date:  1987-03       Impact factor: 4.272

10.  The 'functional' dyad of scorpion toxin Pi1 is not itself a prerequisite for toxin binding to the voltage-gated Kv1.2 potassium channels.

Authors:  Stéphanie Mouhat; Amor Mosbah; Violeta Visan; Heike Wulff; Muriel Delepierre; Hervé Darbon; Stephan Grissmer; Michel De Waard; Jean-Marc Sabatier
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