Literature DB >> 6577440

5-methylcytosine is localized in nucleosomes that contain histone H1.

D J Ball, D S Gross, W T Garrard.   

Abstract

We have developed a procedure for purifying highly specific polyclonal antibodies against 5-methylcytosine. These antibodies were used to probe the distribution of 5-methylcytosine among fractionated nucleosomes of mouse cell chromatin. Our results demonstrate that at least 80% of the 5-methylcytosine is localized in nucleosomes that contain histone H1. Native nucleosomes that lack histone H1 or possess high mobility group proteins package DNA that is 1.6- to 2.3-fold undermethylated. We suggest that the preferential association of methylated sequences with histone H1 has functional significance because DNA methylation has been linked to gene inactivation and histone H1 is known to promote chromatin condensation.

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Year:  1983        PMID： 6577440      PMCID： PMC384283          DOI： 10.1073/pnas.80.18.5490

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  38 in total

1.  The serum proteins in multiple myelomatosis.

Authors:  R A Kekwick
Journal:  Biochem J       Date:  1940-09       Impact factor: 3.857

2.  Properties of active nucleosomes as revealed by HMG 14 and 17 chromatography.

Authors:  S T Weisbrod
Journal:  Nucleic Acids Res       Date:  1982-03-25       Impact factor: 16.971

3.  Isolation of actively transcribed nucleosomes using immobilized HMG 14 and 17 and an analysis of alpha-globin chromatin.

Authors:  S Weisbrod; H Weintraub
Journal:  Cell       Date:  1981-02       Impact factor: 41.582

4.  Varigated chromatin structures of mouse ribosomal RNA genes.

Authors:  A H Davis; T L Reudelhuber; W T Garrard
Journal:  J Mol Biol       Date:  1983-06-15       Impact factor: 5.469

5.  Temperature-sensitive changes in the structure of globin chromatin in lines of red cell precursors transformed by ts-AEV.

Authors:  H Weintraub; H Beug; M Groudine; T Graf
Journal:  Cell       Date:  1982-04       Impact factor: 41.582

6.  Points of contact between histone H1 and the histone octamer.

Authors:  T Boulikas; J M Wiseman; W T Garrard
Journal:  Proc Natl Acad Sci U S A       Date:  1980-01       Impact factor: 11.205

7.  Subunit structures of different electrophoretic forms of nucleosomes.

Authors:  S C Albright; J M Wiseman; R A Lange; W T Garrard
Journal:  J Biol Chem       Date:  1980-04-25       Impact factor: 5.157

8.  Tissue specificity and clustering of methylated cystosines in bovine satellite I DNA.

Authors:  H Sano; R Sager
Journal:  Proc Natl Acad Sci U S A       Date:  1982-06       Impact factor: 11.205

9.  Identification of 5-methylcytosine in DNA fragments immobilized on nitrocellulose paper.

Authors:  H Sano; H D Royer; R Sager
Journal:  Proc Natl Acad Sci U S A       Date:  1980-06       Impact factor: 11.205

10.  Pattern of methylation of two genes coding for housekeeping functions.

Authors:  R Stein; N Sciaky-Gallili; A Razin; H Cedar
Journal:  Proc Natl Acad Sci U S A       Date:  1983-05       Impact factor: 11.205
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  27 in total

Review 1.  DNA methylation and histone deacetylation in the control of gene expression: basic biochemistry to human development and disease.

Authors:  A El-Osta; A P Wolffe
Journal:  Gene Expr       Date:  2000

2.  Histone H1 is dispensable for methylation-associated gene silencing in Ascobolus immersus and essential for long life span.

Authors:  J L Barra; L Rhounim; J L Rossignol; G Faugeron
Journal:  Mol Cell Biol       Date:  2000-01       Impact factor: 4.272

3.  DNA Methylation is Reduced in DNasel-Sensitive Regions of Plant Chromatin.

Authors:  M Klaas; R M Amasino
Journal:  Plant Physiol       Date:  1989-10       Impact factor: 8.340

Review 4.  Biological aspects of cytosine methylation in eukaryotic cells.

Authors:  M Hergersberg
Journal:  Experientia       Date:  1991-12-01

Review 5.  CpG methylation, chromatin structure and gene silencing-a three-way connection.

Authors:  A Razin
Journal:  EMBO J       Date:  1998-09-01       Impact factor: 11.598

6.  Phosphorylation/dephosphorylation of the repressor MDBP-2-H1 selectively affects the level of transcription from a methylated promoter in vitro.

Authors:  A Bruhat; J P Jost
Journal:  Nucleic Acids Res       Date:  1996-05-15       Impact factor: 16.971

7.  The methylated DNA binding protein-2-H1 (MDBP-2-H1) consists of histone H1 subtypes which are truncated at the C-terminus.

Authors:  S Schwarz; D Hess; J P Jost
Journal:  Nucleic Acids Res       Date:  1997-12-15       Impact factor: 16.971

8.  The active immunoglobulin kappa chain gene is packaged by non-ubiquitin-conjugated nucleosomes.

Authors:  S Y Huang; M B Barnard; M Xu; S Matsui; S M Rose; W T Garrard
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

9.  Histone H1 Is required for proper regulation of pyruvate decarboxylase gene expression in Neurospora crassa.

Authors:  H Diego Folco; Michael Freitag; Ana Ramón; Esteban D Temporini; María E Alvarez; Irene García; Claudio Scazzocchio; Eric U Selker; Alberto L Rosa
Journal:  Eukaryot Cell       Date:  2003-04

10.  Demethylation enhances removal of pyrimidine dimers from the overall genome and from specific DNA sequences in Chinese hamster ovary cells.

Authors:  L Ho; V A Bohr; P C Hanawalt
Journal:  Mol Cell Biol       Date:  1989-04       Impact factor: 4.272
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