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Literature DB >> 6524899

Induction of amphotericin B-specific antibodies for use in immunoassays.

J R Little, K D Little, E Plut, M Koldin, G S Kobayashi.

Abstract

High-titered antisera specific for amphotericin B (AmB) were induced by immunization with a protein conjugate of the D-lysyl AmB methyl ester. These polyclonal anti-AmB antibodies reacted preferentially with AmB or the AmB methyl ester and discriminated sharply between nystatin and AmB. A solid-phase radioimmunoassay was developed with radioiodinated immunoglobulin G fractions derived from the anti-AmB antisera. This assay was capable of detecting AmB in the sera in the same concentration range that is regularly achieved during AmB treatment of systemic fungal infections. This study demonstrated the feasibility of immunoassays in measuring the concentration of AmB in blood and tissue fluids.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1984 PMID： 6524899 PMCID： PMC180032 DOI： 10.1128/AAC.26.6.824

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

17 in total

1. Antibody affinity. VII. Multivalent interaction of anti-lactoside antibody.

Authors: P V Gopalakrishnan; F Karush
Journal: J Immunol Date: 1974-09 Impact factor: 5.422

2. A new method of radioiodine labeling of polyanionic macromolecules for radioimmunoassay.

Authors: J R Little; T J Blanke; K D Little
Journal: Eur J Immunol Date: 1976-06 Impact factor: 5.532

Review 3. Antifungal agents useful in therapy of systemic fungal infections.

Authors: G Medoff; J Brajtburg; G S Kobayashi; J Bolard
Journal: Annu Rev Pharmacol Toxicol Date: 1983 Impact factor: 13.820

4. Distribution of doubly radiolabelled amphotericin B methyl ester and amphotericin B in the non-human primate, Macaca mulatta.

Authors: R M Lawrence; P D Hoeprich; F A Jagdis; N Monji; A C Huston; C P Schaffner
Journal: J Antimicrob Chemother Date: 1980-03 Impact factor: 5.790

5. Studies on the absorption, distribution and excretion of radioactivity after intravenous and intraperitoneal administration of 14C-methyl ester of amphotericin B.

Authors: N Monji; D P Bonner; Y Hashimoto; C P Schaffner
Journal: J Antibiot (Tokyo) Date: 1975-04 Impact factor: 2.649

2. Antibodies to nystatin demonstrate polyene sterol specificity and allow immunolabeling of sterols in Saccharomyces cerevisiae.

Authors: H M Walker-Caprioglio; J M MacKenzie; L W Parks
Journal: Antimicrob Agents Chemother Date: 1989-12 Impact factor: 5.191

2 in total

Induction of amphotericin B-specific antibodies for use in immunoassays.

1. Antibody affinity. VII. Multivalent interaction of anti-lactoside antibody.

2. A new method of radioiodine labeling of polyanionic macromolecules for radioimmunoassay.

Review 3. Antifungal agents useful in therapy of systemic fungal infections.

4. Distribution of doubly radiolabelled amphotericin B methyl ester and amphotericin B in the non-human primate, Macaca mulatta.

5. Studies on the absorption, distribution and excretion of radioactivity after intravenous and intraperitoneal administration of 14C-methyl ester of amphotericin B.

6. Amphotericin B methyl ester hydrochloride and amphotericin B: comparative acute toxicity.

7. Polyene macrolide derivatives. 3. Biological properties of polyene macrolide ester salts.

8. Amphotericin B pharmacokinetics in humans.

9. Experimental murine aspergillosis. Comparison of amphotericin B and a new polyene antifungal drug, SCH 28191.

10. N-aminoacyl derivatives of polyene macrolide antibiotics and their esters.

1. A sensitive amphotericin B immunoassay for pharmacokinetic and distribution studies.

2. Antibodies to nystatin demonstrate polyene sterol specificity and allow immunolabeling of sterols in Saccharomyces cerevisiae.