Literature DB >> 6505688

Cloned mycoplasma ribosomal RNA genes for the detection of mycoplasma contamination in tissue cultures.

U B Göbel, E J Stanbridge.   

Abstract

A cloned fragment of the mycoplasma ribosomal RNA operon was used as a molecular probe for the detection of mycoplasmas in cell cultures. According to the conditions of hybridization, the probe can detect prokaryotes in general or mycoplasmas specifically.

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Year:  1984        PMID： 6505688     DOI： 10.1126/science.6505688

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  17 in total

1.  DNA hybridization probe for the Pseudomonas fluorescens group.

Authors:  H Festl; W Ludwig; K H Schleifer
Journal:  Appl Environ Microbiol       Date:  1986-11       Impact factor: 4.792

2.  Comparison of Nucleic Acid Hybridization and Fluorometry for Measurement of the Relationship between RNA/DNA Ratio and Growth Rate in a Marine Bacterium.

Authors:  L Kerkhof; B B Ward
Journal:  Appl Environ Microbiol       Date:  1993-05       Impact factor: 4.792

3.  PCR-based preparation of 23S rRNA-targeted group-specific polynucleotide probes.

Authors:  W Ludwig; S Dorn; N Springer; G Kirchhof; K H Schleifer
Journal:  Appl Environ Microbiol       Date:  1994-09       Impact factor: 4.792

4.  Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR.

Authors:  F J van Kuppeveld; K E Johansson; J M Galama; J Kissing; G Bölske; J T van der Logt; W J Melchers
Journal:  Appl Environ Microbiol       Date:  1994-01       Impact factor: 4.792

5.  Detection and quantitation of Anaplasma marginale in carrier cattle by using a nucleic acid probe.

Authors:  I S Eriks; G H Palmer; T C McGuire; D R Allred; A F Barbet
Journal:  J Clin Microbiol       Date:  1989-02       Impact factor: 5.948

6.  Comparative studies to determine the efficiency of 6 methylpurine deoxyriboside to detect cell culture mycoplasmas.

Authors:  G J McGarrity; H Kotani; D Carson
Journal:  In Vitro Cell Dev Biol       Date:  1986-06

7.  Rate of incorporation of radiolabelled nucleosides does not necessarily reflect the metabolic state of cells in culture: effects of latent mycoplasma contamination.

Authors:  M Merkenschlager; D Kardamakis; F C Rawle; N Spurr; P C Beverley
Journal:  Immunology       Date:  1988-01       Impact factor: 7.397

8.  The retinoblastoma gene functions as a growth and tumor suppressor in human bladder carcinoma cells.

Authors:  R Takahashi; T Hashimoto; H J Xu; S X Hu; T Matsui; T Miki; H Bigo-Marshall; S A Aaronson; W F Benedict
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-15       Impact factor: 11.205

9.  A species-specific DNA probe for the detection of Mycoplasma gallisepticum.

Authors:  M Santha; K Burg; I Rasko; L Stipkovits
Journal:  Infect Immun       Date:  1987-11       Impact factor: 3.441

10.  Clinical and biological characteristics of Ureaplasma urealyticum induced polyarthritis in a patient with common variable hypogammaglobulinaemia.

Authors:  R R Lehmer; B S Andrews; J A Robertson; E J Stanbridge; L de la Maza; G J Friou
Journal:  Ann Rheum Dis       Date:  1991-08       Impact factor: 19.103
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