Physical structure and genetic organisation of the genome of maize streak virus (Kenyan isolate).

The structure of the maize streak virus genome (Kenyan isolate, MSV-K), as determined from the sequence of clones obtained from DNA isolated from virus particles, is composed of one major DNA component of about 2.6 kb. MSV virion DNA is partially double-stranded, composed of a full-length virion (V) strand and a short (70-80b) primer (P) strand. The primer strand has a fixed 5'-end capped with alkaline labile material, presumably 1-2 ribonucleotides. The MSV genome has two major coding regions oriented on opposite strands and flanked by two small intergenic regions. The coding region on the P strand is composed of two major open reading frames (ORFs), arranged in tandem and in the same reading frame. Because the predicted protein derived from a composite of these two ORFs closely corresponds to the product from a single ORF in the cassava latent virus genome, it is likely that this region encodes two proteins with common amino-termini, one a read-through product of the amber codon terminator in the first ORF. The intergenic regions contain potential transcription start and stop signals oriented in the direction of the two opposing coding regions. Considerable DNA sequence heterogeneity was observed, mostly silent or conservative third base substitutions in coding regions and base substitutions, small insertions and small, close-range transpositions in intergenic regions.