Literature DB >> 6322762

Negatively supercoiled DNA from plants infected with a single-stranded DNA virus.

G Sunter, R H Coutts, K W Buck.   

Abstract

A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of -0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzyme.

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Year:  1984        PMID： 6322762     DOI： 10.1016/0006-291x(84)91458-x

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  10 in total

1.  Infectivity and complete nucleotide sequence of the genome of a genetically distinct strain of maize streak virus from Reunion Island.

Authors:  M Peterschmitt; M Granier; R Frutos; B Reynaud
Journal:  Arch Virol       Date:  1996       Impact factor: 2.574

2.  Molecular characterisation of subgenomic single-stranded and double-stranded DNA forms isolated from plants infected with tomato golden mosaic virus.

Authors:  S W MacDowell; R H Coutts; K W Buck
Journal:  Nucleic Acids Res       Date:  1986-10-24       Impact factor: 16.971

3.  Characterisation of multimeric DNA forms associated with tomato golden mosaic virus infection.

Authors:  M J Slomka; K W Buck; R H Coutts
Journal:  Arch Virol       Date:  1988       Impact factor: 2.574

4.  Physical structure and genetic organisation of the genome of maize streak virus (Kenyan isolate).

Authors:  S H Howell
Journal:  Nucleic Acids Res       Date:  1984-10-11       Impact factor: 16.971

5.  DNA and RNA polymerase activities of nuclei and hypotonic extracts of nuclei isolated from tomato golden mosaic virus infected tobacco leaves.

Authors:  R H Coutts; K W Buck
Journal:  Nucleic Acids Res       Date:  1985-11-11       Impact factor: 16.971

6.  Characterisation of DNA forms associated with cassava latent virus infection.

Authors:  J Stanley; R Townsend
Journal:  Nucleic Acids Res       Date:  1985-04-11       Impact factor: 16.971

7.  S1-sensitive sites in the supercoiled double-stranded form of tomato golden mosaic virus DNA component B: identification of regions of potential alternative secondary structure and regulatory function.

Authors:  G Sunter; K W Buck; R H Coutts
Journal:  Nucleic Acids Res       Date:  1985-07-11       Impact factor: 16.971

8.  The nucleotide sequence of cloned wheat dwarf virus DNA.

Authors:  S W MacDowell; H Macdonald; W D Hamilton; R H Coutts; K W Buck
Journal:  EMBO J       Date:  1985-09       Impact factor: 11.598

9.  The nucleotide sequence of maize streak virus DNA.

Authors:  P M Mullineaux; J Donson; B A Morris-Krsinich; M I Boulton; J W Davies
Journal:  EMBO J       Date:  1984-12-20       Impact factor: 11.598

10.  A putative primer for second-strand DNA synthesis of maize streak virus is virion-associated.

Authors:  J Donson; B A Morris-Krsinich; P M Mullineaux; M I Boulton; J W Davies
Journal:  EMBO J       Date:  1984-12-20       Impact factor: 11.598
  10 in total

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