Literature DB >> 6490764

Rapid chromatographic quantitation of glycosylated haemoglobins.

U H Stenman, K Pesonen, K Ylinen, M L Huhtala, K Teramo.   

Abstract

We have developed a rapid chromatographic method for determination of glycosylated haemoglobins by high-performance liquid chromatography with a new cation-exchange column. The haemoglobins are eluted with a three-step gradient in 7 min, and total assay time including re-equilibration of the column is 15 min. The method permits separation and quantitation of HbAlc, even in the presence of elevated levels of HbF. HbA1a, A1b and A2 can also be determined. The results correlate well (r = 0.94) with those obtained by the macro-column method of Trivelli et al. [New Engl. J. Med., 284 (1971) 353] for determination of HbA1c. The method has been fully automated by the use of an automatic injector. The within-assay and between-assay coefficient of variation of the method is 2-3%.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6490764     DOI： 10.1016/s0021-9673(01)89052-x

Source DB:  PubMed          Journal:  J Chromatogr


  10 in total

1.  Acute in vivo effects of insulin on gene expression in adipose tissue in insulin-resistant and insulin-sensitive subjects.

Authors:  J Westerbacka; A Cornér; K Kannisto; M Kolak; J Makkonen; E Korsheninnikova; T Nyman; A Hamsten; R M Fisher; H Yki-Järvinen
Journal:  Diabetologia       Date:  2005-12-16       Impact factor: 10.122

2.  Direct relationship of antepartum glucose control and fetal erythropoietin in human type 1 (insulin-dependent) diabetic pregnancy.

Authors:  J A Widness; K A Teramo; G K Clemons; P Voutilainen; U H Stenman; S M McKinlay; R Schwartz
Journal:  Diabetologia       Date:  1990-06       Impact factor: 10.122

3.  Technetium-99m hexamethylpropylene amine oxime single-photon emission tomography of regional cerebral blood flow in insulin-dependent diabetes.

Authors:  B Keymeulen; K de Metz; R Cluydts; A Bossuyt; G Somers
Journal:  Eur J Nucl Med       Date:  1996-02

4.  Glycosylated haemoglobins in normal pregnancy.

Authors:  K Ylinen; U H Stenman; R Hekali; T Kesäniemi-Kuokkanen; K Teramo
Journal:  Diabetologia       Date:  1985-06       Impact factor: 10.122

5.  Obesity and fat quantification in lean tissues using three-point Dixon MR imaging.

Authors:  Arzu Kovanlikaya; Steven D Mittelman; Andrette Ward; Mitchell E Geffner; Frederick Dorey; Vicente Gilsanz
Journal:  Pediatr Radiol       Date:  2005-03-23

6.  Pregnancy induces nonimmunoglobulin insulin-binding activity in both maternal and cord blood serum.

Authors:  M S Ronkainen; A M Hämäläinen; P Koskela; H K Akerblom; M Knip
Journal:  Clin Exp Immunol       Date:  2001-05       Impact factor: 4.330

7.  Disease-associated autoantibodies during pregnancy and at birth in families affected by type 1 diabetes.

Authors:  A M Hämäläinen; K Savola; P K Kulmala; P Koskela; H K Akerblom; M Knip
Journal:  Clin Exp Immunol       Date:  2001-11       Impact factor: 4.330

8.  Women and men have similar amounts of liver and intra-abdominal fat, despite more subcutaneous fat in women: implications for sex differences in markers of cardiovascular risk.

Authors:  J Westerbacka; A Cornér; M Tiikkainen; M Tamminen; S Vehkavaara; A-M Häkkinen; J Fredriksson; H Yki-Järvinen
Journal:  Diabetologia       Date:  2004-07-28       Impact factor: 10.122

Review 9.  Methods, units and quality requirements for the analysis of haemoglobin A1c in diabetes mellitus.

Authors:  Ilkka Penttilä; Karri Penttilä; Päivi Holm; Harri Laitinen; Päivi Ranta; Jukka Törrönen; Rainer Rauramaa
Journal:  World J Methodol       Date:  2016-06-26

Review 10.  Chromatography of complex protein mixtures.

Authors:  F E Regnier
Journal:  J Chromatogr       Date:  1987-07-17
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.