Literature DB >> 6486790

Purification of Giardia muris cysts by velocity sedimentation.

J F Sauch.   

Abstract

Giardia muris cysts were separated from fecal contaminants in primary isolates by unit gravity velocity sedimentation. Crude isolates obtained by centrifugation over 1.0 M sucrose were overlaid onto a Percoll density gradient, 1.01 to 1.03 g/ml. G. muris cysts were well separated from faster-sedimenting fecal debris and slower-sedimenting Spironucleus muris and bacteria in 1.5 h.

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Mesh：

Year:  1984        PMID： 6486790      PMCID： PMC241540          DOI： 10.1128/aem.48.2.454-455.1984

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  4 in total

1.  Density gradients prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll).

Authors:  H Pertoft; T C Laurent; T Låås; L Kågedal
Journal:  Anal Biochem       Date:  1978-07-15       Impact factor: 3.365

2.  Separation of cells by velocity sedimentation.

Authors:  R G Miller; R A Phillips
Journal:  J Cell Physiol       Date:  1969-06       Impact factor: 6.384

3.  Cell separations by counterflow centrifugation.

Authors:  R J Sanderson; K E Bird
Journal:  Methods Cell Biol       Date:  1977       Impact factor: 1.441

4.  Giardiasis in the mouse: an animal model.

Authors:  I C Roberts-Thomson; D P Stevens; A A Mahmoud; K S Warren
Journal:  Gastroenterology       Date:  1976-07       Impact factor: 22.682
  4 in total
  12 in total

1.  Propidium iodide as an indicator of Giardia cyst viability.

Authors:  J F Sauch; D Flanigan; M L Galvin; D Berman; W Jakubowski
Journal:  Appl Environ Microbiol       Date:  1991-11       Impact factor: 4.792

2.  Comparison of filters for concentrating microbial indicators and pathogens in lake water samples.

Authors:  Donna S Francy; Erin A Stelzer; Amie M G Brady; Carrie Huitger; Rebecca N Bushon; Hon S Ip; Michael W Ware; Eric N Villegas; Vicente Gallardo; H D Alan Lindquist
Journal:  Appl Environ Microbiol       Date:  2012-12-21       Impact factor: 4.792

3.  Differences in staining intensities affect reported occurrences and concentrations of Giardia spp. in surface drinking water sources.

Authors:  K A Alderisio; L F Villegas; M W Ware; L A McDonald; L Xiao; E N Villegas
Journal:  J Appl Microbiol       Date:  2017-10-22       Impact factor: 3.772

4.  Quick and efficient purification of Giardia intestinalis cysts from fecal samples.

Authors:  M E Alvarado; M Wasserman
Journal:  Parasitol Res       Date:  2006-03-24       Impact factor: 2.289

5.  Propagation of Giardia duodenalis cysts in immunosuppressed CF-1 mice.

Authors:  Michael W Ware; Eric N Villegas
Journal:  Vet Parasitol       Date:  2019-03-05       Impact factor: 2.738

6.  Antibody-magnetite method for selective concentration of Giardia lamblia cysts from water samples.

Authors:  J M Bifulco; F W Schaefer
Journal:  Appl Environ Microbiol       Date:  1993-03       Impact factor: 4.792

7.  Determination of Giardia lamblia cyst infective dose for the Mongolian gerbil (Meriones unguiculatus).

Authors:  F W Schaefer; C H Johnson; C H Hsu; E W Rice
Journal:  Appl Environ Microbiol       Date:  1991-08       Impact factor: 4.792

8.  The nucleotide sequence of the entire ribosomal DNA operon and the structure of the large subunit rRNA of Giardia muris.

Authors:  H van Keulen; R R Gutell; S R Campbell; S L Erlandsen; E L Jarroll
Journal:  J Mol Evol       Date:  1992-10       Impact factor: 2.395

9.  Immunofluorescence and morphology of Giardia lamblia cysts exposed to chlorine.

Authors:  J F Sauch; D Berman
Journal:  Appl Environ Microbiol       Date:  1991-05       Impact factor: 4.792

10.  Use of immunofluorescence and phase-contrast microscopy for detection and identification of Giardia cysts in water samples.

Authors:  J F Sauch
Journal:  Appl Environ Microbiol       Date:  1985-12       Impact factor: 4.792
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