Literature DB >> 16565815

Quick and efficient purification of Giardia intestinalis cysts from fecal samples.

M E Alvarado1, M Wasserman.   

Abstract

Giardia intestinalis is an intestinal parasite that has sparked considerable interest because of the public health problem it creates and because it is regarded as one of the earliest divergent eukaryotes. The present report describes a new method for quick, clean, and effective isolation of G. intestinalis cysts from fecal samples. The isolated cysts have the quality required for biochemical studies of the excystation process.

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Year:  2006        PMID： 16565815     DOI： 10.1007/s00436-006-0143-x

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  11 in total

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Authors:  Staffan G Svärd; Per Hagblom; J E Daniel Palm
Journal:  FEMS Microbiol Lett       Date:  2003-01-21       Impact factor: 2.742

2.  Giardia excystation can be induced in vitro in acidic solutions.

Authors:  A K Bingham; E A Meyer
Journal:  Nature       Date:  1979-01-25       Impact factor: 49.962

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Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Phylogenetic meaning of the kingdom concept: an unusual ribosomal RNA from Giardia lamblia.

Authors:  M L Sogin; J H Gunderson; H J Elwood; R A Alonso; D A Peattie
Journal:  Science       Date:  1989-01-06       Impact factor: 47.728

5.  Excystation of in vitro-derived Giardia lamblia cysts.

Authors:  S E Boucher; F D Gillin
Journal:  Infect Immun       Date:  1990-11       Impact factor: 3.441

6.  Purification of Giardia muris cysts by velocity sedimentation.

Authors:  J F Sauch
Journal:  Appl Environ Microbiol       Date:  1984-08       Impact factor: 4.792

7.  A primitive enzyme for a primitive cell: the protease required for excystation of Giardia.

Authors:  W Ward; L Alvarado; N D Rawlings; J C Engel; C Franklin; J H McKerrow
Journal:  Cell       Date:  1997-05-02       Impact factor: 41.582

Review 8.  Giardiasis as a re-emerging infectious disease and its zoonotic potential.

Authors:  R C Thompson
Journal:  Int J Parasitol       Date:  2000-11       Impact factor: 3.981

9.  [Purification and breaking techniques for cysts of Giardia spp].

Authors:  D Polverino; N B Molina; M C Minvielle; M E Lozano; J A Basualdo
Journal:  Rev Argent Microbiol       Date:  2004 Jul-Sep       Impact factor: 1.852

10.  In vitro excystation and subsequent axenic growth of Giardia lamblia.

Authors:  M A Hautus; L M Kortbeek; J C Vetter; J J Laarman
Journal:  Trans R Soc Trop Med Hyg       Date:  1988       Impact factor: 2.184
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  2 in total

1.  Identification and expression of the protein ubiquitination system in Giardia intestinalis.

Authors:  Eva Gallego; Magda Alvarado; Moises Wasserman
Journal:  Parasitol Res       Date:  2007-01-25       Impact factor: 2.289

2.  Calmodulin expression during Giardia intestinalis differentiation and identification of calmodulin-binding proteins during the trophozoite stage.

Authors:  Magda E Alvarado; Moisés Wasserman
Journal:  Parasitol Res       Date:  2011-09-17       Impact factor: 2.289
  2 in total

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