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Literature DB >> 646789

Characterization of the keratan sulphate proteoglycans from bovine corneal stroma.

Abstract

The keratan sulphate proteoglycans that can be prepared from bovine corneal stroma [Axelsson & Heinegård (1975) Biochem. J. 145, 491-500] were characterized by gel chromatography, gel electrophoresis and analytical ultracentrifugation in associative (0.6 M-NaCl) and dissociative (6M-guanidinum chloride) solvents. The proteoglycans aggreagated at low salt concentrations and pH. The weight-average molecular weight of the monomer proteoglycans was established. Keratan sulphate peptides and oligosaccharide peptides were isolated after proteolysis. Their composition indicated that both are linked to protein via asparagine residues. A tentative model for corneal keratan sulphate proteoglycans is suggested.

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Year: 1978 PMID： 646789 PMCID： PMC1183825 DOI： 10.1042/bj1690517

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

30 in total

1. A modified uronic acid carbazole reaction.

Authors: T BITTER; H M MUIR
Journal: Anal Biochem Date: 1962-10 Impact factor: 3.365

2. Studies on corneal polysaccharides. III. Topographic and comparative biochemistry.

Authors: A ANSETH
Journal: Exp Eye Res Date: 1961-12 Impact factor: 3.467

3. The properties of thyroglobulin. I. The effects of alkali.

Authors: H EDELHOCH
Journal: J Biol Chem Date: 1960-05 Impact factor: 5.157

4. Studies on corneal polysaccharides. II. Characterization.

Authors: T C LAURENT; A ANSETH
Journal: Exp Eye Res Date: 1961-12 Impact factor: 3.467

5. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

6. Conformational properties of the complexes formed by proteins and sodium dodecyl sulfate.

Authors: W L Mattice; J M Riser; D S Clark
Journal: Biochemistry Date: 1976-09-21 Impact factor: 3.162

7. Isolation and characterisation of the glycopeptides of the structural glycoprotein of corneal stroma.

Authors: E Moczar; M Moczar; L Robert
Journal: Life Sci Date: 1969-07-15 Impact factor: 5.037

8. Extraction, fractionation and characterization of proteoglycans from bovine tracheal cartilage.

Authors: D Heinegård
Journal: Biochim Biophys Acta Date: 1972-11-28

9. Bovine serum albumin and aqueous guanidine hydrochloride solutions. Preferential and absolute interactions and comparison with other systems.

Authors: E Reisler; Y Haik; H Eisenberg
Journal: Biochemistry Date: 1977-01-25 Impact factor: 3.162

10. Ultrastructural localization of proteoglycans in tissue using cuprolinic blue according to the critical electrolyte concentration method: comparison with biochemical data from the literature.

Authors: T H Van Kuppevelt; T L Rutten; C M Kuyper
Journal: Histochem J Date: 1987-09

Characterization of the keratan sulphate proteoglycans from bovine corneal stroma.

1. A modified uronic acid carbazole reaction.

2. Studies on corneal polysaccharides. III. Topographic and comparative biochemistry.

3. The properties of thyroglobulin. I. The effects of alkali.

4. Studies on corneal polysaccharides. II. Characterization.

5. Protein measurement with the Folin phenol reagent.

6. Conformational properties of the complexes formed by proteins and sodium dodecyl sulfate.

7. Isolation and characterisation of the glycopeptides of the structural glycoprotein of corneal stroma.

8. Extraction, fractionation and characterization of proteoglycans from bovine tracheal cartilage.

9. Bovine serum albumin and aqueous guanidine hydrochloride solutions. Preferential and absolute interactions and comparison with other systems.

10. Distribution of keratan sulfate in cartilage proteoglycans.

1. Studies of a monoclonal antibody to skeletal keratan sulphate. Importance of antibody valency.

Review 2. Proteoglycans in health and disease: structures and functions.

3. Characterization of proteoglycans from the calcified matrix of bovine bone.

4. MT1-MMP cleavage of the antiangiogenic proteoglycan decorin: role in corneal angiogenesis.

5. A novel low-molecular weight chondroitin sulphate proteoglycan isolated from cartilage.

6. Characterization of newly synthesized proteoglycans from rabbit menisci in organ culture.

7. Macular corneal dystrophy: failure to synthesize a mature keratan sulfate proteoglycan.

8. Abnormal product of corneal explants from patients with macular corneal dystrophy.

9. Self-association of dermatan sulphate proteoglycans from bovine sclera.

10. Ultrastructural localization of proteoglycans in tissue using cuprolinic blue according to the critical electrolyte concentration method: comparison with biochemical data from the literature.