Literature DB >> 6417454

Plasmid transformation in Bacillus subtilis. Effects of the insertion of Bacillus subtilis rRNA genes into plasmids.

A Iglesias, P Ceglowski, T A Trautner.   

Abstract

Two HindIII generated DNA fragments of 3.0 and 2.3 Kb derived from rRNA genes of B. subtilis were cloned in E. coli with pBR322. The 3.0 Kb fragment could be subcloned in B. subtilis using pC194. However, only the multimeric, but not the monomeric derivatives of this hybrid plasmid were active in transformation of B. subtilis cells. The 2.3 Kb fragment could neither be subcloned in pC194 nor in pPL603, using both cell and protoplast transformation. We attribute the nonclonability of the 2.3 Kb fragment in B. subtilis to the presence of strong promoter activity in this fragment. Direct proof for the presence of a strong promoter, which is apparently responsible for the transcription of the rRNA gene, was obtained in experiments with B. subtilis and E. coli promoter search plasmids.

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Year:  1983        PMID： 6417454     DOI： 10.1007/BF00327660

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  31 in total

1.  A freeze-squeeze method for recovering long DNA from agarose gels.

Authors:  R W Thuring; J P Sanders; P Borst
Journal:  Anal Biochem       Date:  1975-05-26       Impact factor: 3.365

2.  Different specific activities of the monomeric and oligomeric forms of plasmid DNA in transformation of B. subtilis and E. coli.

Authors:  M Mottes; G Grandi; V Sgaramella; U Canosi; G Morelli; T A Trautner
Journal:  Mol Gen Genet       Date:  1979-07-24

3.  The genome of Bacillus subtilis phage SPP1: the arrangement of restriction endonuclease generated fragments.

Authors:  S W Ratcliff; J Luh; A T Ganesan; B Behrens; R Thompson; M A Montenegro; G Morelli; T A Trautner
Journal:  Mol Gen Genet       Date:  1979-01-10

4.  The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis.

Authors:  U Canosi; G Morelli; T A Trautner
Journal:  Mol Gen Genet       Date:  1978-11-09

5.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

6.  Structure and organization of a cluster of sic tRNA genes in the space between tandem ribosomal RNA gene sets in Bacillus subtilis.

Authors:  E F Wawrousek; J N Hansen
Journal:  J Biol Chem       Date:  1983-01-10       Impact factor: 5.157

7.  Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region.

Authors:  S Moriya; N Ogasawara; H Yoshikawa
Journal:  Nucleic Acids Res       Date:  1985-04-11       Impact factor: 16.971

8.  Detailed physical mapping of the ribosomal RNA genes of Bacillus subtilis.

Authors:  G C Stewart; F E Wilson; K F Bott
Journal:  Gene       Date:  1982-09       Impact factor: 3.688

9.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

10.  Plasmid transformation in Bacillus subtilis: effects of insertion of Bacillus subtilis DNA into plasmid pC194.

Authors:  U Canosi; A Iglesias; T A Trautner
Journal:  Mol Gen Genet       Date:  1981
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  12 in total

1.  Usefulness of the ID32 staph system and a method based on rRNA gene restriction site polymorphism analysis for species and subspecies identification of staphylococcal clinical isolates.

Authors:  O Chesneau; S Aubert; A Morvan; J L Guesdon; N el Solh
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948

2.  Evaluation and comparison of molecular techniques for epidemiological typing of Salmonella enterica subsp. enterica serovar dublin.

Authors:  B Liebisch; S Schwarz
Journal:  J Clin Microbiol       Date:  1996-03       Impact factor: 5.948

3.  Genetic and Phenotypic Diversity of Bacillus polymyxa in Soil and in the Wheat Rhizosphere.

Authors:  P Mavingui; G Laguerre; O Berge; T Heulin
Journal:  Appl Environ Microbiol       Date:  1992-06       Impact factor: 4.792

4.  Recognition of Leuconostoc oenos strains by the use of DNA restriction profiles.

Authors:  M Lamoureux; H Prévost; J F Cavin; C Diviès
Journal:  Appl Microbiol Biotechnol       Date:  1993-07       Impact factor: 4.813

5.  Methicillin-resistant Staphylococcus aureus isolates recovered from a New York City hospital: analysis by molecular fingerprinting techniques.

Authors:  H de Lencastre; A de Lencastre; A Tomasz
Journal:  J Clin Microbiol       Date:  1996-09       Impact factor: 5.948

6.  Mapping of rRNA genes with integrable plasmids in Bacillus subtilis.

Authors:  G LaFauci; R L Widom; R L Eisner; E D Jarvis; R Rudner
Journal:  J Bacteriol       Date:  1986-01       Impact factor: 3.490

7.  Characterization of Vibrio fischeri rRNA operons and subcloning of a ribosomal DNA promoter.

Authors:  D Amikam; J Kuhn
Journal:  J Bacteriol       Date:  1987-05       Impact factor: 3.490

8.  Methicillin-resistant Staphylococcus aureus disease in a Portuguese hospital: characterization of clonal types by a combination of DNA typing methods.

Authors:  H de Lencastre; I Couto; I Santos; J Melo-Cristino; A Torres-Pereira; A Tomasz
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1994-01       Impact factor: 3.267

9.  Plasmid transformation in Bacillus subtilis: factors affecting the synapsis of donor and recipient DNA.

Authors:  P Ceglowski; T A Trautner
Journal:  Mol Gen Genet       Date:  1984

10.  Phenotypic and genomic variation among Staphylococcus epidermidis strains infecting joint prostheses.

Authors:  J O Galdbart; A Morvan; N Desplaces; N el Solh
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948
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