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Literature DB >> 6392014

Specific deletion occurring in the directed evolution of 6-phosphogluconate dehydrogenase in Escherichia coli.

R D Miller, D E Dykhuizen, L Green, D L Hartl.

Abstract

A novel genetic change leading to increased activity of 6-phosphogluconate dehydrogenase (6PGD) in E. coli has been observed. The mutation is a deletion of approximately 0.4 kilobase pairs occurring between the structural gene of 6PGD (gnd) and one copy of an insertion element (IS5) found normally in E. coli K12 a few hundred base pairs upstream (counterclockwise) from gnd at 44 minutes on the conventional genetic map. The deletion is associated with a threefold higher activity of 6PGD and a 57% increase in the maximum growth rate when cells are grown in gluconate.

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Year: 1984 PMID： 6392014 PMCID： PMC1224263

Source DB: PubMed Journal: Genetics ISSN： 0016-6731 Impact factor: 4.562

8 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

3. Growth-rate-dependent alteration of 6-phosphogluconate dehydrogenase and glucose 6-phosphate dehydrogenase levels in Escherichia coli K-12.

Authors: R E Wolf; D M Prather; F M Shea
Journal: J Bacteriol Date: 1979-09 Impact factor: 3.490

Review 4. Compilation and analysis of Escherichia coli promoter DNA sequences.

Authors: D K Hawley; W R McClure
Journal: Nucleic Acids Res Date: 1983-04-25 Impact factor: 16.971

5. Structural analysis of insertion sequence IS5.

Authors: M Kröger; G Hobom
Journal: Nature Date: 1982-05-13 Impact factor: 49.962

6. Molecular cloning, correlation of genetic and restriction maps, and determination of the direction of transcription of gnd of Escherichia coli.

Authors: M S Nasoff; R E Wolf
Journal: J Bacteriol Date: 1980-08 Impact factor: 3.490

7. Selective neutrality of 6PGD allozymes in E. coli and the effects of genetic background.

Authors: D Dykhuizen; D L Hartl
Journal: Genetics Date: 1980-12 Impact factor: 4.562

8. Escherichia coli K-12 F' plasmids carrying insertion sequences IS1 and IS5.

Authors: B Schoner
Journal: Gene Date: 1983-03 Impact factor: 3.688

8 in total

5 in total

Specific deletion occurring in the directed evolution of 6-phosphogluconate dehydrogenase in Escherichia coli.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

3. Growth-rate-dependent alteration of 6-phosphogluconate dehydrogenase and glucose 6-phosphate dehydrogenase levels in Escherichia coli K-12.

Review 4. Compilation and analysis of Escherichia coli promoter DNA sequences.

5. Structural analysis of insertion sequence IS5.

6. Molecular cloning, correlation of genetic and restriction maps, and determination of the direction of transcription of gnd of Escherichia coli.

7. Selective neutrality of 6PGD allozymes in E. coli and the effects of genetic background.

8. Escherichia coli K-12 F' plasmids carrying insertion sequences IS1 and IS5.

1. Mutations enhancing amino acid catabolism confer a growth advantage in stationary phase.

2. Growth-rate-dependent expression and cloning of gnd alleles from natural isolates of Escherichia coli.

3. Plasmid macro-evolution: selection of deletions during adaptation in a nutrient-limited environment.

4. Limits of adaptation: the evolution of selective neutrality.

5. Adaptive evolution that requires multiple spontaneous mutations. I. Mutations involving an insertion sequence.