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Literature DB >> 6374620

Oligonucleotide mutagenesis of the lacPUV5 promoter.

L M Munson, W Mandecki, M H Caruthers, W S Reznikoff.

Abstract

Synthetic oligonucleotides were used to introduce mutations into the lacPUV5 promoter. Four mutations were obtained at positions -13, -14, and -15, with respect to the transcriptional start site. The effects of these mutations were measured in vivo and the results are discussed with respect to the consensus sequence and other promoter mutations located in this region.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1984 PMID： 6374620 PMCID： PMC318807 DOI： 10.1093/nar/12.9.4011

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

13 in total

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Authors: M L Berman; A Landy
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

Review 2. Regulatory sequences involved in the promotion and termination of RNA transcription.

Authors: M Rosenberg; D Court
Journal: Annu Rev Genet Date: 1979 Impact factor: 16.830

3. Naturally occurring promoter down mutation: nucleotide sequence of the trp promoter/operator/leader region of Shigella dysenteriae 16.

Authors: G Miozzari; C Yanofsky
Journal: Proc Natl Acad Sci U S A Date: 1978-11 Impact factor: 11.205

Review 4. Compilation and analysis of Escherichia coli promoter DNA sequences.

Authors: D K Hawley; W R McClure
Journal: Nucleic Acids Res Date: 1983-04-25 Impact factor: 16.971

5. Mutations in the Escherichia coli operon that define two promoters and the binding site of the cyclic AMP receptor protein.

Authors: S Busby; H Aiba; B de Crombrugghe
Journal: J Mol Biol Date: 1982-01-15 Impact factor: 5.469

6. The regulatory region of the divergent argECBH operon in Escherichia coli K-12.

Authors: J Piette; R Cunin; A Boyen; D Charlier; M Crabeel; F Van Vliet; N Glansdorff; C Squires; C L Squires
Journal: Nucleic Acids Res Date: 1982-12-20 Impact factor: 16.971

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Authors: U Siebenlist; R B Simpson; W Gilbert
Journal: Cell Date: 1980-06 Impact factor: 41.582

8. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

9. Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA.

Authors: M J Zoller; M Smith
Journal: Nucleic Acids Res Date: 1982-10-25 Impact factor: 16.971

5. Interaction between E. coli RNA polymerase and the tetR promoter from pSC101: homologies and differences with other E. coli promoter systems from close contact point studies.

Authors: G Duval-Valentin; R Ehrlich
Journal: Nucleic Acids Res Date: 1986-03-11 Impact factor: 16.971

6. Site specific demethylation in the promoter of human gamma-globin gene does not alleviate methylation mediated suppression.

Authors: E J Murray; F Grosveld
Journal: EMBO J Date: 1987-08 Impact factor: 11.598

7. Transcription initiation by mix and match elements: flexibility for polymerase binding to bacterial promoters.

Authors: India G Hook-Barnard; Deborah M Hinton
Journal: Gene Regul Syst Bio Date: 2007

7 in total