Literature DB >> 323416

Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses.

M F Clark, A N Adams.   

Abstract

Some characteristics of a microplate method for the detection and assay of plant viruses using enzyme-labelled antibodies are described. The method enabled the highly sensitive detection of a number of morphologically different viruses in purified preparations and in unclarified extracts of herbaceous hosts and of infected crop plants. Virus concentrations were estimated by photometric measurement of the colour intensity of the hydrolysed substrate. The suitability of the tehcnique for various field and research applications is considered.

Entities:  

Mesh：

Year:  1977        PMID： 323416     DOI： 10.1099/0022-1317-34-3-475

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  289 in total

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Journal:  Proc Natl Acad Sci U S A       Date:  1996-02-20       Impact factor: 11.205

9.  In situ localisation of beet necrotic yellow vein virus (BNYVV) in rootlets of susceptible and resistant beet plants.

Authors:  O E Scholten; H Paul; D Peters; J W Van Lent; R W Goldbach
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10.  Gene I mutants of peanut chlorotic streak virus, a caulimovirus, replicate in plants but do not move from cell to cell.

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