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Literature DB >> 6368221

Cloning and sequencing of the preprotoxin-coding region of the yeast M1 double-stranded RNA.

Abstract

Complementary DNA (cDNA) copies of the M1-1, toxin-coding region of the yeast M1 double-stranded RNA (dsRNA) have been cloned and sequenced. These sequences, in combination with the known terminal sequence of M1-1 dsRNA, identify a translation reading frame for a 316 amino acid protein of 34.7 kd, similar in size to the preprotoxin produced from M1 dsRNA by in vitro translation. Potential glycosylation sites in the preprotoxin peptide are identified. Based on its methionine content the extracellular yeast toxin appears to be contained within the C-terminal region of the precursor.

Entities: Chemical

Mesh：

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Year: 1984 PMID： 6368221 PMCID： PMC557305 DOI： 10.1002/j.1460-2075.1984.tb01769.x

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

35 in total

1. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors: M Grunstein; D S Hogness
Journal: Proc Natl Acad Sci U S A Date: 1975-10 Impact factor: 11.205

2. Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor.

Authors: S Nakanishi; A Inoue; T Kita; M Nakamura; A C Chang; S N Cohen; S Numa
Journal: Nature Date: 1979-03-29 Impact factor: 49.962

3. Carbohydrate-peptide linkage in glycoproteins.

Authors: J P Aubert; G Biserte; M H Loucheux-Lefebvre
Journal: Arch Biochem Biophys Date: 1976-08 Impact factor: 4.013

4. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

5. Peptide chain conformation and the glycosylation of glycoproteins.

Authors: J G Beeley
Journal: Biochem Biophys Res Commun Date: 1977-06-20 Impact factor: 3.575

Review 6. Prediction of the secondary structure of proteins from their amino acid sequence.

Authors: P Y Chou; G D Fasman
Journal: Adv Enzymol Relat Areas Mol Biol Date: 1978

7. Yeast killer toxin: purification and characterisation of the protein toxin from Saccharomyces cerevisiae.

Authors: R G Palfree; H Bussey
Journal: Eur J Biochem Date: 1979-02-01

8. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.

Authors: F Bolivar; R L Rodriguez; M C Betlach; H W Boyer
Journal: Gene Date: 1977 Impact factor: 3.688

9. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

10. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

Authors: G Blobel; B Dobberstein
Journal: J Cell Biol Date: 1975-12 Impact factor: 10.539

26 in total

1. Kinetics of ribosomal pausing during programmed -1 translational frameshifting.

Authors: J D Lopinski; J D Dinman; J A Bruenn
Journal: Mol Cell Biol Date: 2000-02 Impact factor: 4.272

Review 2. Yeast killer systems.

Authors: W Magliani; S Conti; M Gerloni; D Bertolotti; L Polonelli
Journal: Clin Microbiol Rev Date: 1997-07 Impact factor: 26.132

Cloning and sequencing of the preprotoxin-coding region of the yeast M1 double-stranded RNA.

1. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

2. Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor.

3. Carbohydrate-peptide linkage in glycoproteins.

4. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

5. Peptide chain conformation and the glycosylation of glycoproteins.

Review 6. Prediction of the secondary structure of proteins from their amino acid sequence.

7. Yeast killer toxin: purification and characterisation of the protein toxin from Saccharomyces cerevisiae.

8. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.

9. DNA sequencing with chain-terminating inhibitors.

10. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

1. Kinetics of ribosomal pausing during programmed -1 translational frameshifting.

Review 2. Yeast killer systems.

3. The [KIL-d] element specifically regulates viral gene expression in yeast.

4. In vivo mapping of a sequence required for interference with the yeast killer virus.

5. Functions of conserved motifs in the RNA-dependent RNA polymerase of a yeast double-stranded RNA virus.

Review 6. Yeast killer toxin K1 and its exploitation in genetic manipulations.

7. Conservative replication and transcription of Saccharomyces cerevisiae viral double-stranded RNA in vitro.

8. Conserved regions in defective interfering viral double-stranded RNAs from a yeast virus.

9. Structure and expression of the M2 genomic segment of a type 2 killer virus of yeast.

10. Secretion and processing of insulin precursors in yeast.