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Literature DB >> 6366514

Isolation and characterization of the centromere from chromosome V (CEN5) of Saccharomyces cerevisiae.

Abstract

We have cloned a functional centromeric DNA sequence from Saccharomyces cerevisiae. Using the 2 mu chromosome-loss mapping technique and meiotic tetrad analysis, we have identified this DNA sequence as the centromere of chromosome V (CEN5). The CEN5 sequence has been localized on an 1,100-base-pair BamHI-BglII restriction fragment. Plasmids containing CEN5 and an autonomously replicating sequence are mitotically stable in S. cerevisiae and segregate in a Mendelian fashion during meiosis.

Entities: Species

Mesh：

Substances：
DNA, Fungal

Year: 1984 PMID： 6366514 PMCID： PMC368661 DOI： 10.1128/mcb.4.1.86-91.1984

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

25 in total

1. Isolation of yeast DNA.

Authors: D R Cryer; R Eccleshall; J Marmur
Journal: Methods Cell Biol Date: 1975 Impact factor: 1.441

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

4. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors: H C Birnboim; J Doly
Journal: Nucleic Acids Res Date: 1979-11-24 Impact factor: 16.971

5. Isolation and characterisation of a yeast chromosomal replicator.

Authors: D T Stinchcomb; K Struhl; R W Davis
Journal: Nature Date: 1979-11-01 Impact factor: 49.962

6. Transformation in Escherichia coli: cryogenic preservation of competent cells.

Authors: D A Morrison
Journal: J Bacteriol Date: 1977-10 Impact factor: 3.490

7. Genetic mapping in Saccharomyces.

Authors: R K Mortimer; D C Hawthorne
Journal: Genetics Date: 1966-01 Impact factor: 4.562

8. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

Authors: K Struhl; D T Stinchcomb; S Scherer; R W Davis
Journal: Proc Natl Acad Sci U S A Date: 1979-03 Impact factor: 11.205

9. Transformation of yeast.

Authors: A Hinnen; J B Hicks; G R Fink
Journal: Proc Natl Acad Sci U S A Date: 1978-04 Impact factor: 11.205

10. Centromeric DNA from chromosome VI in Saccharomyces cerevisiae strains.

Authors: L Panzeri; P Philippsen
Journal: EMBO J Date: 1982 Impact factor: 11.598

31 in total

1. The phenotype of the minichromosome maintenance mutant mcm3 is characteristic of mutants defective in DNA replication.

Authors: S I Gibson; R T Surosky; B K Tye
Journal: Mol Cell Biol Date: 1990-11 Impact factor: 4.272

8. Single base-pair mutations in centromere element III cause aberrant chromosome segregation in Saccharomyces cerevisiae.

Authors: J McGrew; B Diehl; M Fitzgerald-Hayes
Journal: Mol Cell Biol Date: 1986-02 Impact factor: 4.272

9. Toxic effects of excess cloned centromeres.

Authors: B Futcher; J Carbon
Journal: Mol Cell Biol Date: 1986-06 Impact factor: 4.272

10. Tightly centromere-linked gene (SPO15) essential for meiosis in the yeast Saccharomyces cerevisiae.

Authors: E Yeh; J Carbon; K Bloom
Journal: Mol Cell Biol Date: 1986-01 Impact factor: 4.272