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Literature DB >> 334731

Transformation in Escherichia coli: cryogenic preservation of competent cells.

Abstract

Escherichia coli cells prepared for transformation by treatment with cold 0.1 M CaCl2 remained viable and competent after storage at -82 degrees C in 15% glycerol; thawed-cell samples yielded up to 10(6) transformants per microgram of plasmid deoxyribonucleic acid.

Entities: Chemical Species

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Year: 1977 PMID： 334731 PMCID： PMC221863 DOI： 10.1128/jb.132.1.349-351.1977

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

11 in total

1. ON THE MECHANISM OF DEOXYRIBONUCLEATE INTEGRATION IN PNEUMOCOCCAL TRANSFORMATION.

Authors: M S FOX; M K ALLEN
Journal: Proc Natl Acad Sci U S A Date: 1964-08 Impact factor: 11.205

2. Transduction of linked genetic characters of the host by bacteriophage P1.

Authors: E S LENNOX
Journal: Virology Date: 1955-07 Impact factor: 3.616

3. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.

Authors: E M Lederberg; S N Cohen
Journal: J Bacteriol Date: 1974-09 Impact factor: 3.490

4. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors: D B Clewell
Journal: J Bacteriol Date: 1972-05 Impact factor: 3.490

5. Number of transformable units per cell in Diplococcus pneumoniae.

Authors: R D Porter; W R Guild
Journal: J Bacteriol Date: 1969-03 Impact factor: 3.490

6. Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex.

Authors: D Dubnau; R Davidoff-Abelson
Journal: J Mol Biol Date: 1971-03-14 Impact factor: 5.469

7. Biochemical construction of specific chimeric plasmids from ColE1 DNA and unfractionated Escherichia coli DNA.

Authors: C J Collins; D A Jackson; A J DeVries
Journal: Proc Natl Acad Sci U S A Date: 1976-11 Impact factor: 11.205

8. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors: S N Cohen; A C Chang; L Hsu
Journal: Proc Natl Acad Sci U S A Date: 1972-08 Impact factor: 11.205

9. Genetic transformation in Escherichia coli K12.

Authors: S D Cosloy; M Oishi
Journal: Proc Natl Acad Sci U S A Date: 1973-01 Impact factor: 11.205

10. EFFECT OF INTERSPECIFIC TRANSFORMATION ON LINKAGE RELATIONSHIPS OF MARKERS IN HAEMOPHILUS INFLUENZAE AND HAEMOPHILUS PARAINFLUENZAE.

Authors: L NICKEL; S H GOODGAL
Journal: J Bacteriol Date: 1964-12 Impact factor: 3.490

102 in total

1. The phenotype of the minichromosome maintenance mutant mcm3 is characteristic of mutants defective in DNA replication.

Authors: S I Gibson; R T Surosky; B K Tye
Journal: Mol Cell Biol Date: 1990-11 Impact factor: 4.272

2. The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter.

Authors: M Bun-Ya; M Nishimura; S Harashima; Y Oshima
Journal: Mol Cell Biol Date: 1991-06 Impact factor: 4.272

3. Interallelic complementation of dnaE(Ts) mutations.

Authors: S K Bryan; R E Moses
Journal: J Bacteriol Date: 1992-07 Impact factor: 3.490

4. Cholera toxin B-subunit gene fusion: structural and functional analysis of the chimeric protein.

Authors: M T Dertzbaugh; D L Peterson; F L Macrina
Journal: Infect Immun Date: 1990-01 Impact factor: 3.441

5. Extension of the host range of Escherichia coli vectors by incorporation of RSF1010 replication and mobilization functions.

Authors: U B Priefer; R Simon; A Pühler
Journal: J Bacteriol Date: 1985-07 Impact factor: 3.490

Review 6. Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell.

Authors: Axel A Ekman; Jian-Hua Chen; Jessica Guo; Gerry McDermott; Mark A Le Gros; Carolyn A Larabell
Journal: Biol Cell Date: 2016-11-14 Impact factor: 4.458

7. Cloning of genes determining the production of mannose-resistant fimbriae in a uropathogenic strain of Escherichia coli belonging to serogroup O6.

Authors: S Clegg
Journal: Infect Immun Date: 1982-11 Impact factor: 3.441

8. Expression and regulation of the penicillin G acylase gene from Proteus rettgeri cloned in Escherichia coli.

Authors: G O Daumy; J A Williams; A S McColl; T J Zuzel; D Danley
Journal: J Bacteriol Date: 1986-10 Impact factor: 3.490

9. Structure of two pectate lyase genes from Erwinia chrysanthemi EC16 and their high-level expression in Escherichia coli.

Authors: N T Keen; S Tamaki
Journal: J Bacteriol Date: 1986-11 Impact factor: 3.490

10. Intrachromosomal movement of genetically marked Saccharomyces cerevisiae transposons by gene conversion.

Authors: G S Roeder; M Smith; E J Lambie
Journal: Mol Cell Biol Date: 1984-04 Impact factor: 4.272