| Literature DB >> 2076551 |
J J Heus1, B J Zonneveld, H Y Steensma, J A Van den Berg.
Abstract
A direct selection method was used to isolate centromeres from a genomic library of the yeast Kluyveromyces lactis. The method is based on the lethality at high copy number of the ochre-suppressing tRNA gene SUP11. Five different chromosomal fragments were found that confer mitotic stability to plasmids containing a replication origin of K. lactis (KARS). In addition, KARS plasmids containing these fragments have a copy number of approximately one, and each of the five fragments hybridizes to a different chromosome of K. lactis. From these results we conclude that five of the six centromeres of K. lactis have been isolated. These centromeres do not function in S. cerevisiae.Entities:
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Year: 1990 PMID: 2076551 DOI: 10.1007/BF00327022
Source DB: PubMed Journal: Curr Genet ISSN: 0172-8083 Impact factor: 3.886