Literature DB >> 6360046

DNA-DNA hybridization assay for detection of Salmonella spp. in foods.

R Fitts, M Diamond, C Hamilton, M Neri.   

Abstract

We have developed a DNA-DNA hybridization test for the presence of Salmonella spp. in foods. This test requires an initial pre-enrichment of food samples in nutrient broth but does not require selective enrichment. Samples of food cultures are collected on membrane filters and assayed by molecular hybridization to labeled probes. The probes consist of DNA sequences which are unique to the genus Salmonella and are widely distributed in the genus. A diverse panel of foods was assayed successfully by this methodology.

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Year:  1983        PMID: 6360046      PMCID: PMC239532          DOI: 10.1128/aem.46.5.1146-1151.1983

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  11 in total

1.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors:  M Grunstein; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  Electrical impedance measurements: rapid method for detecting and monitoring microorganisms.

Authors:  P Cady; S W Dufour; J Shaw; S J Kraeger
Journal:  J Clin Microbiol       Date:  1978-03       Impact factor: 5.948

4.  Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.

Authors:  J R Broach; J N Strathern; J B Hicks
Journal:  Gene       Date:  1979-12       Impact factor: 3.688

5.  Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA.

Authors:  J Brandsma; G Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

6.  An assay for the detection of the DNA genome of hepatitis B virus in serum.

Authors:  M Berninger; M Hammer; B Hoyer; J L Gerin
Journal:  J Med Virol       Date:  1982       Impact factor: 2.327

7.  Rapid detection of Salmonella spp. in food by use of the ISO-GRID hydrophobic grid membrane filter.

Authors:  P Entis; M H Brodsky; A N Sharpe; G A Jarvis
Journal:  Appl Environ Microbiol       Date:  1982-02       Impact factor: 4.792

8.  A membrane-filter technique for the detection of complementary DNA.

Authors:  D T Denhardt
Journal:  Biochem Biophys Res Commun       Date:  1966-06-13       Impact factor: 3.575

9.  Detection of enterotoxigenic Escherichia coli by DNA colony hybridization.

Authors:  S L Moseley; I Huq; A R Alim; M So; M Samadpour-Motalebi; S Falkow
Journal:  J Infect Dis       Date:  1980-12       Impact factor: 5.226

10.  Rapid identification of Leishmania species by specific hybridization of kinetoplast DNA in cutaneous lesions.

Authors:  D F Wirth; D M Pratt
Journal:  Proc Natl Acad Sci U S A       Date:  1982-11       Impact factor: 11.205

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  40 in total

1.  Sensitive enzyme-amplified electrical immunoassay for protein A-bearing Staphylococcus aureus in foods.

Authors:  J L Brooks; B Mirhabibollahi; R G Kroll
Journal:  Appl Environ Microbiol       Date:  1990-11       Impact factor: 4.792

2.  A semi-homogeneous amperometric immunosensor for protein A-bearing Staphylococcus aureus in foods.

Authors:  B Mirhabibollahi; J L Brooks; R G Kroll
Journal:  Appl Microbiol Biotechnol       Date:  1990-11       Impact factor: 4.813

3.  Identification of lotus rhizobia by direct DNA hybridization of crushed root nodules.

Authors:  J E Cooper; A J Bjourson; J K Thompson
Journal:  Appl Environ Microbiol       Date:  1987-07       Impact factor: 4.792

4.  Evaluation of a fluorescence-labelled oligonucleotide probe targeting 23S rRNA for in situ detection of Salmonella serovars in paraffin-embedded tissue sections and their rapid identification in bacterial smears.

Authors:  S Nordentoft; H Christensen; H C Wegener
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

5.  Construction and applications of DNA probes for detection of polychlorinated biphenyl-degrading genotypes in toxic organic-contaminated soil environments.

Authors:  S Walia; A Khan; N Rosenthal
Journal:  Appl Environ Microbiol       Date:  1990-01       Impact factor: 4.792

Review 6.  Impact of molecular biology on the detection of foodborne pathogens.

Authors:  P Feng
Journal:  Mol Biotechnol       Date:  1997-06       Impact factor: 2.695

7.  Detection of foodborne pathogens using DNA probes and a dipstick format.

Authors:  E P Groody
Journal:  Mol Biotechnol       Date:  1996-12       Impact factor: 2.695

8.  Bacterial chromosomal restriction endonuclease analysis of the homology of Bacteroides species.

Authors:  W C Bradbury; R G Murray; C Mancini; V L Morris
Journal:  J Clin Microbiol       Date:  1985-01       Impact factor: 5.948

9.  Probability of recovering pathogenic Escherichia coli from foods.

Authors:  W E Hill; J L Ferreira; W L Payne; V M Jones
Journal:  Appl Environ Microbiol       Date:  1985-06       Impact factor: 4.792

10.  DNA-based diagnostic tests for Salmonella species targeting agfA, the structural gene for thin, aggregative fimbriae.

Authors:  J L Doran; S K Collinson; J Burian; G Sarlós; E C Todd; C K Munro; C M Kay; P A Banser; P I Peterkin; W W Kay
Journal:  J Clin Microbiol       Date:  1993-09       Impact factor: 5.948

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