Literature DB >> 16347400

Identification of lotus rhizobia by direct DNA hybridization of crushed root nodules.

J E Cooper1, A J Bjourson, J K Thompson.   

Abstract

Hybridization of crushed Lotus pedunculatus root nodules with P-labeled total genomic DNA probes was used to identify Rhizobium loti and Bradyrhizobium sp. (Lotus rhizobia). Probes always hybridized with homologous target DNA and frequently with DNAs of other strains from the same genus. Intergeneric hybridization did not occur. Results were comparable to those from colony hybridization.

Entities:  

Year:  1987        PMID： 16347400      PMCID： PMC203937          DOI： 10.1128/aem.53.7.1705-1707.1987

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  4 in total

1.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors:  M Grunstein; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  DNA-DNA hybridization assay for detection of Salmonella spp. in foods.

Authors:  R Fitts; M Diamond; C Hamilton; M Neri
Journal:  Appl Environ Microbiol       Date:  1983-11       Impact factor: 4.792

4.  C(4)-dicarboxylate transport mutants of Rhizobium trifolii form ineffective nodules on Trifolium repens.

Authors:  C W Ronson; P Lyttleton; J G Robertson
Journal:  Proc Natl Acad Sci U S A       Date:  1981-07       Impact factor: 11.205
  4 in total
  8 in total

1.  Identification of frankia strains by direct DNA hybridization of crushed nodules.

Authors:  P Simonet; N T Le; E T Du Cros; R Bardin
Journal:  Appl Environ Microbiol       Date:  1988-10       Impact factor: 4.792

2.  A Positive Strain Identification Method for Rhizobium meliloti.

Authors:  R Wheatcroft; R J Watson
Journal:  Appl Environ Microbiol       Date:  1988-02       Impact factor: 4.792

3.  DNA Hybridization Probe for Use in Determining Restricted Nodulation among Bradyrhizobium japonicum Serocluster 123 Field Isolates.

Authors:  M J Sadowsky; P B Cregan; H H Keyser
Journal:  Appl Environ Microbiol       Date:  1990-06       Impact factor: 4.792

4.  Isolation of Rhizobium loti Strain-Specific DNA Sequences by Subtraction Hybridization.

Authors:  A J Bjourson; J E Cooper
Journal:  Appl Environ Microbiol       Date:  1988-11       Impact factor: 4.792

5.  Identification of inoculant strains and naturalized populations ofRhizobium leguminosarum bvtrifolii using complementary methodologies.

Authors:  E Fabiano; A Arias
Journal:  World J Microbiol Biotechnol       Date:  1990-06       Impact factor: 3.312

6.  Identification of Vibrio anguillarum in fish by using partial 16S rRNA sequences and a specific 16S rRNA oligonucleotide probe.

Authors:  A S Rehnstam; A Norqvist; H Wolf-Watz; A Hagström
Journal:  Appl Environ Microbiol       Date:  1989-08       Impact factor: 4.792

7.  Combined subtraction hybridization and polymerase chain reaction amplification procedure for isolation of strain-specific Rhizobium DNA sequences.

Authors:  A J Bjourson; C E Stone; J E Cooper
Journal:  Appl Environ Microbiol       Date:  1992-07       Impact factor: 4.792

8.  The use of lacZ marker in enumeration of Azotobacter chroococcum in carrier based inoculants.

Authors:  Manu Solanki; Faquir C Garg
Journal:  Braz J Microbiol       Date:  2014-08-29       Impact factor: 2.476
  8 in total

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