Literature DB >> 6347990

The use of monoclonal antibodies against thyroglobulin in immunoperoxidase techniques.

W Ormanns, R Schäffer.   

Abstract

A monoclonal mouse antibody to human thyroglobulin was used in a modified biotin-peroxidase-complex method to demonstrate thyroglobulin in different tissues and tumors of the thyroid gland. Mouse ascites fluid containing monoclonal antibodies could be diluted up to 1:1,600 for detection of thyroglobulin, whereas no staining was observed in a medullary carcinoma. Nonspecific background staining was negligible. These results demonstrate that monoclonal antibodies against thyroglobulin are suitable in the immunohistochemical localisation of thyroid hormones.

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Year:  1983        PMID： 6347990     DOI： 10.1007/bf00489496

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  5 in total

1.  Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.

Authors:  S M Hsu; L Raine; H Fanger
Journal:  J Histochem Cytochem       Date:  1981-04       Impact factor: 2.479

2.  A simple method for incubation of tissue sections in immunohistochemistry.

Authors:  W Ormanns; U Pfeifer
Journal:  Histochemistry       Date:  1981

3.  Immunoperoxidase techniques: practical and theoretical aspects.

Authors:  C R Taylor
Journal:  Arch Pathol Lab Med       Date:  1978-03       Impact factor: 5.534

4.  Background staining and sensitivity of the unlabelled antibody-enzyme (PAP) method. Comparison with the peroxidase labelled antibody sandwich method using formalin fixed paraffin embedded material.

Authors:  J Burns
Journal:  Histochemistry       Date:  1975-06-05

5.  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors:  R C Graham; M J Karnovsky
Journal:  J Histochem Cytochem       Date:  1966-04       Impact factor: 2.479
  5 in total
  1 in total

1.  The immunohistochemical demonstration of thyroglobulin in human thyroid tumour xenografts using a monoclonal antibody.

Authors:  T C Richardson; S A Humm; T E Carr
Journal:  Histochemistry       Date:  1987
  1 in total

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