Literature DB >> 50308

Background staining and sensitivity of the unlabelled antibody-enzyme (PAP) method. Comparison with the peroxidase labelled antibody sandwich method using formalin fixed paraffin embedded material.

J Burns.   

Abstract

The PAP procedure was compared with the peroxidase labelled antibody sandwich method and was found to be at least 20 times more sensitive. Background staining was reduced by the addition of normal swine serum to all the immune sera or by pretreating sections with it at the beginning of either method. The PAP procedure could be effectively reduced to a period of 1 hour or less.

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Year:  1975        PMID： 50308     DOI： 10.1007/bf00499711

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  9 in total

1.  Letter: The use of formalin fixation in an improved method for immunofluorescence histochemical investigation of neurophysin in the hypothalamus.

Authors:  W B Watkins; J J Evans
Journal:  J Histochem Cytochem       Date:  1974-02       Impact factor: 2.479

2.  The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody.

Authors:  C R Taylor; J Burns
Journal:  J Clin Pathol       Date:  1974-01       Impact factor: 3.411

3.  Letter: Destruction of endogenous peroxidase activity in order to locate cellular antigens by peroxidase-labeled antibodies.

Authors:  A Pitts; E E Williams
Journal:  J Histochem Cytochem       Date:  1974-01       Impact factor: 2.479

4.  Immunoperoxidase and immunofluorescence techniques in pig tissues.

Authors:  P Brown; J Bourne; M Steel
Journal:  Histochemistry       Date:  1974

5.  An immunoperoxidase technique for the identification of gastrin-producing cells.

Authors:  J Piris; R Whitehead
Journal:  J Clin Pathol       Date:  1974-10       Impact factor: 3.411

6.  Localization of lysozyme in normal rat tissues by an immunoperoxidase method.

Authors:  M Klockars; E F Osserman
Journal:  J Histochem Cytochem       Date:  1974-03       Impact factor: 2.479

7.  Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol.

Authors:  J G Streefkerk
Journal:  J Histochem Cytochem       Date:  1972-10       Impact factor: 2.479

8.  The role of cathepsin D in the pathogenesis of tuberculosis. A histochemical study employing unlabeled antibodies and the peroxidase-antiperoxidase complex.

Authors:  O Rojas-Espinosa; A M Dannenberg; L A Sternberger; T Tsuda
Journal:  Am J Pathol       Date:  1974-01       Impact factor: 4.307

9.  Intracellular immunoglobulins. A comparative study on three standard tissue processing methods using horseradish peroxidase and fluorochrome conjugates.

Authors:  J Burns; M Hambridge; C R Taylor
Journal:  J Clin Pathol       Date:  1974-07       Impact factor: 3.411
  9 in total
  75 in total

1.  Immunoperoxidase localisation of hepatitis B antigen (HB) in formalin-paraffin processed liver tissue.

Authors:  J Burns
Journal:  Histochemistry       Date:  1975-07-30

2.  [Unusual location of extramedullary plasmacytomas (author's transl)].

Authors:  A Schmitt-Gräff; W Arnold
Journal:  Arch Otorhinolaryngol       Date:  1979

3.  Restricted feeding and human intestinal plasma cell development.

Authors:  W F Knox
Journal:  Arch Dis Child       Date:  1986-08       Impact factor: 3.791

4.  Incidence in South-west Scotland of hepatitis B surface antigen in the liver of patients with hepatocellular carcinoma.

Authors:  M L Turbitt; R S Patrick; R B Goudie; W M Buchanan
Journal:  J Clin Pathol       Date:  1977-12       Impact factor: 3.411

5.  Immunohistochemical demonstration of alpha-1-antitrypsin in the islet cells of human pancreas.

Authors:  M B Ray; V J Desmet
Journal:  Cell Tissue Res       Date:  1978-02-14       Impact factor: 5.249

6.  Application of the peroxidase-antiperoxidase immunoassay to the identification of Salmonellae from pure culture and animal tissue.

Authors:  C M McRill; T T Kramer; R W Griffith
Journal:  J Clin Microbiol       Date:  1984-08       Impact factor: 5.948

7.  Immunoglobulin-containing cells in human tonsils as demonstrated by immunohistochemistry.

Authors:  R C Curran; E L Jones
Journal:  Clin Exp Immunol       Date:  1977-04       Impact factor: 4.330

8.  The effects of prednisolone in leucocyte function in man. A double blind controlled study.

Authors:  J R Clarke; R F Gagnon; F M Gotch; M R Heyworth; I C Maclennan; S C Truelove; C A Waller
Journal:  Clin Exp Immunol       Date:  1977-05       Impact factor: 4.330

9.  Fibronectin in the synovium of chronic inflammatory joint disease.

Authors:  V Mayston; P I Mapp; P G Davies; P A Revell
Journal:  Rheumatol Int       Date:  1984       Impact factor: 2.631

10.  Immunoglobulin-containing plasma cells in chronic parotitis and malignant lymphomas of the parotid gland. Comparing immunocytochemical observations of frequency and localization.

Authors:  C von Gumberz; G Seifert
Journal:  Virchows Arch A Pathol Anat Histol       Date:  1980
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