Literature DB >> 6331614

Benzo(B)thiophene-2-carboxylic acid: calcium uptake and cyclic AMP production in isolated bone cells.

J C Robin, M J Brown, N Weinfeld, R M Dziak.   

Abstract

The purpose of the present study was to investigate the mechanism of action on bone of Benzo(B)Thiophene-2-Carboxylic Acid (BL-5583). BL-5583, at a dose range of 0.01-100 micrograms/ml, inhibited spontaneous as well as A23187 and PTH-induced bone resorption in tissue culture. This compound also decreased calcium uptake in both osteoclastic and osteoblastic enriched bone cell populations obtained by sequential collagenase digestion of 1-2 day newborn rat calvariae. The decrease occurred after a 5 min. incubation with 45Ca and BL-5583. The effective dose range was 0.01-100 micrograms/ml. No effect on leucine incorporation or lactic acid production by bone cells was observed. BL-5583 also induced a transient decrease in calcium uptake in skin cells isolated from fetal rats by collagenase digestion, suggesting a lack of tissue specificity for this compound. No effect on cyclic AMP in isolated bone cells was observed with the same dose range that produced a calcium effect.

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Year:  1984        PMID： 6331614     DOI： 10.1007/bf02405317

Source DB:  PubMed          Journal:  Calcif Tissue Int        ISSN： 0171-967X            Impact factor:   4.333


  17 in total

1.  Calcitonin--a new hormone from the pa rathyroid which lowers blood calcium.

Authors:  D H COPP
Journal:  Oral Surg Oral Med Oral Pathol       Date:  1963-07

2.  Effects of vitamin D3 metabolites on bone cell calcium transport.

Authors:  R Dziak
Journal:  Calcif Tissue Res       Date:  1978-11-10

3.  2-Thiophenecarboxylic acid: a hypoglycemic, antilipolytic agent with hypocalcemic and hypophosphatemic effects in rats.

Authors:  W Lloyd; V S Fang; H Wells; A H Tashjian
Journal:  Endocrinology       Date:  1969-10       Impact factor: 4.736

4.  A sensitive in vitro method for studying the induction and inhibition of bone resorption.

Authors:  J J Reynolds; J T Dingle
Journal:  Calcif Tissue Res       Date:  1970

5.  Calcium-mediated enhancement of the cyclic AMP response in cultured bone cells.

Authors:  W A Peck; G Kohler; S Barr
Journal:  Calcif Tissue Int       Date:  1981       Impact factor: 4.333

6.  Calcium transport in isolated bone cells. III. Effects of parathyroid hormone and cyclic 3',5'-AMP.

Authors:  R Dziak; P H Stern
Journal:  Endocrinology       Date:  1975-11       Impact factor: 4.736

7.  Thyrocalcitonin: inhibitor of bone resorption in tissue culture.

Authors:  J Friedman; L G Raisz
Journal:  Science       Date:  1965-12-10       Impact factor: 47.728

8.  Biochemical characterization with parathormone and calcitonin of isolated bone cells: provisional identification of osteoclasts and osteoblasts.

Authors:  R A Luben; G L Wong; D V Cohn
Journal:  Endocrinology       Date:  1976-08       Impact factor: 4.736

9.  Studies on osteoporosis IV. Effect of benzo[b]thiophene-2- and dibenzothiophene-4-carboxylic acid on heparin accelerated osteoporosis.

Authors:  J C Robin; S D Sharma; K Francis; M Rosenstein; R Moore; J A Vida; C C Thomas; J L Ambrus
Journal:  J Med       Date:  1980

10.  2-Thiophenecarboxylic acid: inhibitor of bone resorption in tissue culture.

Authors:  V S Fang; C Minkin; P Goldhaber
Journal:  Science       Date:  1971-04-09       Impact factor: 47.728
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  1 in total

1.  Effects of thionaphthene 2-carboxylic acid and related compounds on bone resorption in organ culture.

Authors:  L G Raisz; C Alander; C Onkelinx; G A Rodan
Journal:  Calcif Tissue Int       Date:  1985-09       Impact factor: 4.333
  1 in total

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