Literature DB >> 6330698

Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals.

B L Semler, A J Dorner, E Wimmer.   

Abstract

Sub-genomic cDNA clones representing the entire genomic RNA of poliovirus Type 1 (Mahoney) have been isolated in E. coli. Construction of a complete cDNA copy of the poliovirus genome in the EcoRI site of plasmid vector pBR325 from these clones is described. Introduction of plasmid DNA containing the complete cDNA copy of polio RNA into cultured primate cells by transfection produces infectious poliovirus. The virus produced by such a transfection appears to be identical to wild type poliovirus. Isolation of a polio recombinant plasmid containing SV40 transcription and replication signals is also described. Transfection of COS-1 cells with this plasmid yields greater than 1,600 plaque-forming units (PFU) per microgram of input DNA.

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Year:  1984        PMID： 6330698      PMCID： PMC318905          DOI： 10.1093/nar/12.12.5123

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  46 in total

1.  Function of simian virus 40 gene A in transforming infection.

Authors:  P Tegtmeyer
Journal:  J Virol       Date:  1975-03       Impact factor: 5.103

2.  Characteristics of a human cell line transformed by DNA from human adenovirus type 5.

Authors:  F L Graham; J Smiley; W C Russell; R Nairn
Journal:  J Gen Virol       Date:  1977-07       Impact factor: 3.891

3.  A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA.

Authors:  G N Godson; D Vapnek
Journal:  Biochim Biophys Acta       Date:  1973-04-11

4.  Requirement of 3'-terminal poly(adenylic acid) for the infectivity of poliovirus RNA.

Authors:  D H Spector; D Baltimore
Journal:  Proc Natl Acad Sci U S A       Date:  1974-08       Impact factor: 11.205

5.  Calcium-dependent bacteriophage DNA infection.

Authors:  M Mandel; A Higa
Journal:  J Mol Biol       Date:  1970-10-14       Impact factor: 5.469

6.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

7.  Polyadenylic acid at the 3'-terminus of poliovirus RNA.

Authors:  Y Yogo; E Wimmer
Journal:  Proc Natl Acad Sci U S A       Date:  1972-07       Impact factor: 11.205

8.  Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.

Authors:  D L Kacian; J C Myers
Journal:  Proc Natl Acad Sci U S A       Date:  1976-07       Impact factor: 11.205

9.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

10.  Isolation of a viral polypeptide associated with poliovirus RNA polymerase.

Authors:  R E Lundquist; E Ehrenfeld; J V Maizel
Journal:  Proc Natl Acad Sci U S A       Date:  1974-12       Impact factor: 11.205
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  57 in total

1.  High frequency of single-base transitions and extreme frequency of precise multiple-base reversion mutations in poliovirus.

Authors:  J C de la Torre; C Giachetti; B L Semler; J J Holland
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-01       Impact factor: 11.205

2.  Enzymatic activity of poliovirus RNA polymerase mutants with single amino acid changes in the conserved YGDD amino acid motif.

Authors:  S A Jablonski; M Luo; C D Morrow
Journal:  J Virol       Date:  1991-09       Impact factor: 5.103

3.  trans rescue of a mutant poliovirus RNA polymerase function.

Authors:  W A Charini; C C Burns; E Ehrenfeld; B L Semler
Journal:  J Virol       Date:  1991-05       Impact factor: 5.103

4.  A mutant poliovirus containing a novel proteolytic cleavage site in VP3 is altered in viral maturation.

Authors:  W S Blair; S S Hwang; M F Ypma-Wong; B L Semler
Journal:  J Virol       Date:  1990-04       Impact factor: 5.103

5.  Characterization of poliovirus clones containing lethal and nonlethal mutations in the genome-linked protein VPg.

Authors:  Q Reuer; R J Kuhn; E Wimmer
Journal:  J Virol       Date:  1990-06       Impact factor: 5.103

6.  Limited expression of poliovirus by vaccinia virus recombinants due to inhibition of the vector by proteinase 2A.

Authors:  J E Jewell; L A Ball; R Rueckert
Journal:  J Virol       Date:  1990-03       Impact factor: 5.103

Review 7.  Genetic engineering of animal RNA viruses.

Authors:  K K Conzelmann; G Meyers
Journal:  Trends Microbiol       Date:  1996-10       Impact factor: 17.079

8.  Construction of less neurovirulent polioviruses by introducing deletions into the 5' noncoding sequence of the genome.

Authors:  N Iizuka; M Kohara; K Hagino-Yamagishi; S Abe; T Komatsu; K Tago; M Arita; A Nomoto
Journal:  J Virol       Date:  1989-12       Impact factor: 5.103

9.  Coinfection with recombinant vaccinia viruses expressing poliovirus P1 and P3 proteins results in polyprotein processing and formation of empty capsid structures.

Authors:  D C Ansardi; D C Porter; C D Morrow
Journal:  J Virol       Date:  1991-04       Impact factor: 5.103

10.  Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo.

Authors:  Susanne Pfefferle; Verena Krähling; Vanessa Ditt; Klaus Grywna; Elke Mühlberger; Christian Drosten
Journal:  Virol J       Date:  2009-08-24       Impact factor: 4.099
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