Literature DB >> 59925

Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.

D L Kacian, J C Myers.   

Abstract

The synthesis of large, possibly complete, complementary DNA (cDNA) copies of poliovirus RNA by avian myeloblastosis virus DNA polymerase is described. The cDNA consists of two size classes, the larger of which is approximately 7500 nucleotides. In the presence of excess deoxynucleoside triphosphates, ribonucleoside triphosphates, or sodium pyrophosphate, only the larger material is obtained. Yields of the large cDNA are 50-75% of the input RNA.

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Year:  1976        PMID： 59925      PMCID： PMC430492          DOI： 10.1073/pnas.73.7.2191

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  26 in total

1.  A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.

Authors:  F Sanger; A R Coulson
Journal:  J Mol Biol       Date:  1975-05-25       Impact factor: 5.469

2.  SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.

Authors:  F W STUDIER
Journal:  J Mol Biol       Date:  1965-02       Impact factor: 5.469

3.  Site-specific cleavage of single-stranded DNA by a Hemophilus restriction endonuclease.

Authors:  K Horiuchi; N D Zinder
Journal:  Proc Natl Acad Sci U S A       Date:  1975-07       Impact factor: 11.205

Review 4.  Restriction endonucleases in the analysis and restructuring of dna molecules.

Authors:  D Nathans; H O Smith
Journal:  Annu Rev Biochem       Date:  1975       Impact factor: 23.643

Review 5.  RNA-directed DNA polymerase--properties and functions in oncogenic RNA viruses and cells.

Authors:  M Green; G F Gerard
Journal:  Prog Nucleic Acid Res Mol Biol       Date:  1974

6.  Isolation of poly(adenylic acid)-rich ribonucleic acid from mouse myeloma and synthesis of complementary deoxyribonucleic acid.

Authors:  C H Faust; H Diggelmann; B Mach
Journal:  Biochemistry       Date:  1973-02-27       Impact factor: 3.162

7.  Molecular weight of poliovirus ribonucleic acid.

Authors:  N Granboulan; M Girard
Journal:  J Virol       Date:  1969-10       Impact factor: 5.103

8.  Studies on the RNA from avian myeloblastosis virus.

Authors:  R L Erikson
Journal:  Virology       Date:  1969-01       Impact factor: 3.616

9.  Analysis of the ribonucleic acid of murine leukemia virus.

Authors:  J P Bader; T L Steck
Journal:  J Virol       Date:  1969-10       Impact factor: 5.103

10.  Isolation and properties of poliovirus minus strand ribonucleic acid.

Authors:  P Roy; D H Bishop
Journal:  J Virol       Date:  1970-11       Impact factor: 5.103
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  52 in total

1.  Detection of enteroviruses in cell cultures by using in situ transcription.

Authors:  J M Carstens; S Tracy; N M Chapman; C J Gauntt
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948

2.  Reverse transcriptase. The use of cloned Moloney murine leukemia virus reverse transcriptase to synthesize DNA from RNA.

Authors:  G F Gerard; D K Fox; M Nathan; J M D'Alessio
Journal:  Mol Biotechnol       Date:  1997-08       Impact factor: 2.695

3.  Degradation of cellular mRNA during infection by herpes simplex virus.

Authors:  Y Nishioka; S Silverstein
Journal:  Proc Natl Acad Sci U S A       Date:  1977-06       Impact factor: 11.205

4.  Cloning of cDNA sequences for an Artemia salina hnRNP protein: evidence for conservation through evolution.

Authors:  M Cruz-Alvarez; W Szer; A Pellicer
Journal:  Nucleic Acids Res       Date:  1985-06-11       Impact factor: 16.971

5.  Alterations in the protein synthetic apparatus of Friend erythroleukemia cells infected with vesicular stomatitis virus or herpes simplex virus.

Authors:  Y Nishioka; S Silverstein
Journal:  J Virol       Date:  1978-01       Impact factor: 5.103

6.  Effects of cDNA hybridization on translation of encephalomyocarditis virus RNA.

Authors:  D S Shih; I W Park; C L Evans; J M Jaynes; A C Palmenberg
Journal:  J Virol       Date:  1987-06       Impact factor: 5.103

7.  Construction of a cDNA to the hamster CAD gene and its application toward defining the domain for aspartate transcarbamylase.

Authors:  K Shigesada; G R Stark; J A Maley; L A Niswander; J N Davidson
Journal:  Mol Cell Biol       Date:  1985-07       Impact factor: 4.272

8.  Calcitonin mRNA polymorphism: peptide switching associated with alternative RNA splicing events.

Authors:  M G Rosenfeld; C R Lin; S G Amara; L Stolarsky; B A Roos; E S Ong; R M Evans
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

9.  Abundant androgen regulated mRNAs in mouse submandibular gland: cell-free translation of renin precursor mRNA.

Authors:  R E Pratt; V J Dzau; A J Ouellette
Journal:  Nucleic Acids Res       Date:  1981-07-24       Impact factor: 16.971

10.  Epstein-Barr virus RNA VII: size and direction of transcription of virus-specified cytoplasmic RNAs in a transformed cell line.

Authors:  V van Santen; A Cheung; E Kieff
Journal:  Proc Natl Acad Sci U S A       Date:  1981-03       Impact factor: 11.205
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