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Literature DB >> 4373729

Isolation of a viral polypeptide associated with poliovirus RNA polymerase.

Abstract

Poliovirus-infected HeLa cells were labeled with radioactive methionine or phenylalanine and subjected to a new purification procedure for the viral induced RNA polymerase activity. Detergent-solubilized polymerase activity was purified by precipitation with 2 M LiCl and sedimentation through sucrose gradients. Approximately 0.001% of the incorporated amino acid radio-activity sediments with the peak of polymerase activity. Gradient fractions comprising the polymerase activity peak were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found to contain predominantly one virus-specific polypeptide. Polyacrylamide gel electrophoresis also reveals that this purified polypeptide migrates with a 58,000 molecular weight noncapsid polio-virus polypeptide.

Entities: Chemical Species

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Year: 1974 PMID： 4373729 PMCID： PMC433979 DOI： 10.1073/pnas.71.12.4773

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

23 in total

1. STUDIES ON EMC VIRAL RNA SYNTHESIS AND ITS LOCALIZATION IN INFECTED KREBS ASCITES CELLS.

Authors: L DALGARNO; E M MARTIN
Journal: Virology Date: 1965-07 Impact factor: 3.616

2. The isolation of two enzyme-ribonucleic acid complexes involved in the synthesis of foot-and-mouth disease virus ribonucleic acid.

Authors: R B Arlinghaus; J Polatnick
Journal: Proc Natl Acad Sci U S A Date: 1969-03 Impact factor: 11.205

3. Polypeptide cleavages in the formation of poliovirus proteins.

Authors: M F Jacobson; D Baltimore
Journal: Proc Natl Acad Sci U S A Date: 1968-09 Impact factor: 11.205

4. Evidence for differences in size and composition of the poliovirus-specific polypeptides in infected HeLa cells.

Authors: J V Maizel; D F Summers
Journal: Virology Date: 1968-09 Impact factor: 3.616

5. Evidence for large precursor proteins in poliovirus synthesis.

Authors: D F Summers; J V Maizel
Journal: Proc Natl Acad Sci U S A Date: 1968-03 Impact factor: 11.205

6. Action of guanidine on the replication of poliovirus RNA.

Authors: L A Caliguiri; I Tamm
Journal: Virology Date: 1968-07 Impact factor: 3.616

7. Specific cleavage of viral proteins as steps in the synthesis and maturation of enteroviruses.

Authors: J J Holland; E D Kiehn
Journal: Proc Natl Acad Sci U S A Date: 1968-07 Impact factor: 11.205

8. Aspects of the synthesis of poliovirus RNA and the formation of virus particles.

Authors: D Baltimore; M Girard; J E Darnell
Journal: Virology Date: 1966-06 Impact factor: 3.616

9. Effects on host cell metabolism following synchronous infection with poliovirus.

Authors: S Penman; D Summers
Journal: Virology Date: 1965-12 Impact factor: 3.616

10. Membranous structures associated with translation and transcription of poliovirus RNA.

Authors: L A Caliguiri; I Tamm
Journal: Science Date: 1969-11-14 Impact factor: 47.728

26 in total

1. Strand-specific RNA synthesis defects in a poliovirus with a mutation in protein 3A.

Authors: Natalya L Teterina; Mario S Rinaudo; Ellie Ehrenfeld
Journal: J Virol Date: 2003-12 Impact factor: 5.103

2. Protease required for processing picornaviral coat protein resides in the viral replicase gene.

Authors: A C Palmenberg; M A Pallansch; R R Rueckert
Journal: J Virol Date: 1979-12 Impact factor: 5.103

3. Proteolysis of noncapsid protein 2 of type 3 poliovirus at the restrictive temperature: breakdown of noncapsid protein 2 correlates with loss of RNA synthesis.

Authors: S A Bowles; D R Tershak
Journal: J Virol Date: 1978-08 Impact factor: 5.103

Isolation of a viral polypeptide associated with poliovirus RNA polymerase.

1. STUDIES ON EMC VIRAL RNA SYNTHESIS AND ITS LOCALIZATION IN INFECTED KREBS ASCITES CELLS.

2. The isolation of two enzyme-ribonucleic acid complexes involved in the synthesis of foot-and-mouth disease virus ribonucleic acid.

3. Polypeptide cleavages in the formation of poliovirus proteins.

4. Evidence for differences in size and composition of the poliovirus-specific polypeptides in infected HeLa cells.

5. Evidence for large precursor proteins in poliovirus synthesis.

6. Action of guanidine on the replication of poliovirus RNA.

7. Specific cleavage of viral proteins as steps in the synthesis and maturation of enteroviruses.

8. Aspects of the synthesis of poliovirus RNA and the formation of virus particles.

9. Effects on host cell metabolism following synchronous infection with poliovirus.

10. Membranous structures associated with translation and transcription of poliovirus RNA.

1. Strand-specific RNA synthesis defects in a poliovirus with a mutation in protein 3A.

2. Protease required for processing picornaviral coat protein resides in the viral replicase gene.

3. Proteolysis of noncapsid protein 2 of type 3 poliovirus at the restrictive temperature: breakdown of noncapsid protein 2 correlates with loss of RNA synthesis.

4. Role of a viral membrane polypeptide in strand-specific initiation of poliovirus RNA synthesis.

5. Structural organization of poliovirus RNA replication is mediated by viral proteins of the P2 genomic region.

6. Replication-competent picornaviruses with complete genomic RNA 3' noncoding region deletions.

7. Synthesis of plus- and minus-strand RNA from poliovirion RNA template in vitro.

8. Poliovirus polyuridylic acid polymerase and RNA replicase have the same viral polypeptide.

9. Identification of poliovirus polypeptide P63 as a soluble RNA-dependent RNA polymerase.

10. Poliovirus-specific primer-dependent RNA polymerase able to copy poly(A).