Literature DB >> 6330042

Host-dependent transposon Tn5-mediated streptomycin resistance.

G F De Vos, T M Finan, E R Signer, G C Walker.   

Abstract

Transposon Tn5 encodes streptomycin resistance in addition to kanamycin-neomycin resistance. This resistance was not detectable in Escherichia coli but was efficiently expressed in Rhizobium meliloti and certain other strains. By analysis of cloned Tn5 restriction endonuclease fragments, the streptomycin resistance (str) gene was located in the right-hand side of the central region as the transposon is conventionally drawn. Transcription of str appeared to originate at pL, the promoter for the neo gene (neomycin phosphotransferase type II). Expression of streptomycin resistance in E. coli was obtained after cloning of the neo-str region downstream of a strong E. coli promoter. A construct in which PL was deleted also showed differential expression of streptomycin resistance.

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Year:  1984        PMID： 6330042      PMCID： PMC215646          DOI： 10.1128/jb.159.1.395-399.1984

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  28 in total

1.  A freeze-squeeze method for recovering long DNA from agarose gels.

Authors:  R W Thuring; J P Sanders; P Borst
Journal:  Anal Biochem       Date:  1975-05-26       Impact factor: 3.365

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Authors:  A W Johnston; J E Beringer
Journal:  J Gen Microbiol       Date:  1975-04

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Authors:  R A Jorgensen; S J Rothstein; W S Reznikoff
Journal:  Mol Gen Genet       Date:  1979

4.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

5.  Complete nucleotide sequence of the Escherichia coli plasmid pBR322.

Authors:  J G Sutcliffe
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1979

6.  R factor transfer in Rhizobium leguminosarum.

Authors:  J E Beringer
Journal:  J Gen Microbiol       Date:  1974-09

7.  The inverted repeats of Tn5 are functionally different.

Authors:  S J Rothstein; R A Jorgensen; K Postle; W S Reznikoff
Journal:  Cell       Date:  1980-03       Impact factor: 41.582

8.  Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.

Authors:  D H Figurski; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

9.  Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.

Authors:  F Bolivar; R L Rodriguez; M C Betlach; H W Boyer
Journal:  Gene       Date:  1977       Impact factor: 3.688

10.  Transposition of R factor genes to bacteriophage lambda.

Authors:  D E Berg; J Davies; B Allet; J D Rochaix
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205
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  13 in total

Review 1.  [Contribution to the discussion on the release of transgenic organisms. II. Ecologic aspects].

Authors:  K Wöhrmann
Journal:  Naturwissenschaften       Date:  1991-05

2.  Expression of Tn5-encoded streptomycin resistance in E. coli.

Authors:  P Mazodier; O Genilloud; E Giraud; F Gasser
Journal:  Mol Gen Genet       Date:  1986-09

3.  Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes.

Authors:  P Mazodier; P Cossart; E Giraud; F Gasser
Journal:  Nucleic Acids Res       Date:  1985-01-11       Impact factor: 16.971

4.  Lipopolysaccharide mutants of Rhizobium meliloti are not defective in symbiosis.

Authors:  R H Clover; J Kieber; E R Signer
Journal:  J Bacteriol       Date:  1989-07       Impact factor: 3.490

5.  Isolation and characterization of symbiotic mutants of bradyrhizobium sp. (Arachis) strain NC92: mutants with host-specific defects in nodulation and nitrogen fixation.

Authors:  K J Wilson; V Anjaiah; P T Nambiar; F M Ausubel
Journal:  J Bacteriol       Date:  1987-05       Impact factor: 3.490

6.  Genetic manipulations in Rhizobium meliloti utilizing two new transposon Tn5 derivatives.

Authors:  G F De Vos; G C Walker; E R Signer
Journal:  Mol Gen Genet       Date:  1986-09

7.  Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes.

Authors:  T M Finan; B Kunkel; G F De Vos; E R Signer
Journal:  J Bacteriol       Date:  1986-07       Impact factor: 3.490

8.  Genetic analysis of the Rhizobium meliloti bacA gene: functional interchangeability with the Escherichia coli sbmA gene and phenotypes of mutants.

Authors:  A Ichige; G C Walker
Journal:  J Bacteriol       Date:  1997-01       Impact factor: 3.490

9.  Bleomycin increases amikacin and streptomycin resistance in Escherichia coli harboring transposon Tn5.

Authors:  J Blazquez; J L Martinez; F Baquero
Journal:  Antimicrob Agents Chemother       Date:  1993-09       Impact factor: 5.191

10.  General transduction in Rhizobium meliloti.

Authors:  T M Finan; E Hartweig; K LeMieux; K Bergman; G C Walker; E R Signer
Journal:  J Bacteriol       Date:  1984-07       Impact factor: 3.490
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