| Literature DB >> 6329684 |
J H Xin, S Kvist, B Dobberstein.
Abstract
A cDNA clone known to code for a mouse histocompatibility (class I) antigen was found to contain a sequence specific for a subpopulation of H-2 genes. This unique sequence is located in the 3' non-coding region close to the stretch of poly(A) nucleotides. A subclone containing this fragment (pH-2d-5) has been used to select hybridizing mRNA. Translation of the mRNA in vitro shows that H-2Kd mRNA is selected. Southern blot analysis of DNA from congenic recombinant mice show that at least one gene containing this sequence is located at the K locus (region) of the major histocompatibility complex. This gene contains a 3.7-kb BglII and a 13-kb EcoRI restriction endonuclease fragment. This gene has been isolated from a genomic DNA library.Entities:
Mesh:
Substances:
Year: 1982 PMID: 6329684 PMCID: PMC553069 DOI: 10.1002/j.1460-2075.1982.tb01192.x
Source DB: PubMed Journal: EMBO J ISSN: 0261-4189 Impact factor: 11.598