Literature DB >> 573889

Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels.

N Rave, R Crkvenjakov, H Boedtker.   

Abstract

Poly A containing RNA isolated from embryonic chick calvaria was transferred from 6% formaldehyde 0.75% agarose gels to diazobenzyloxymethyl paper and the paper then hybridized to either nick translated pro alpha 1 collagen cDNA clones, pCg1 or pCg54, or to the nick translated pro alpha 2 collagen cDNA clone, pCg45. From the mobilities of the bands hybridizing most strongly to each, pro alpha 2 collagen mRNA was shown to be slightly larger than pro alpha 1 mRNA; they are 5100 and 4900 nucleotides long respectively. pCg54 also hybridized weakly to two bands of lower mobility, corresponding to RNAs 6.4 and 5.6 kb long. Neither pCg54 nor pCg45 hybridized to type II procollagen mRNA in poly A containing RNA isolated from embryonic chick sterna.

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Year:  1979        PMID： 573889      PMCID： PMC327956          DOI： 10.1093/nar/6.11.3559

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  6 in total

Review 1.  The primary structure of collagen.

Authors:  P P Fietzek; K Kühn
Journal:  Int Rev Connect Tissue Res       Date:  1976

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

Authors:  H Lehrach; D Diamond; J M Wozney; H Boedtker
Journal:  Biochemistry       Date:  1977-10-18       Impact factor: 3.162

4.  Construction and characterization of a 2.5-kilobase procollagen clone.

Authors:  H Lehrach; A M Frischauf; D Hanahan; J Wozney; F Fuller; R Crkvenjakov; H Boedtker; P Doty
Journal:  Proc Natl Acad Sci U S A       Date:  1978-11       Impact factor: 11.205

5.  Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

Authors:  J C Alwine; D J Kemp; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

6.  Procollagen complementary DNA, a probe for messenger RNA purification and the number of type I collagen genes.

Authors:  A M Frischauf; H Lehrach; C Rosner; H Boedtker
Journal:  Biochemistry       Date:  1978-08-08       Impact factor: 3.162
  6 in total
  203 in total

1.  Coevolution of transcriptional and allosteric regulation at the chorismate metabolic branch point of Saccharomyces cerevisiae.

Authors:  S Krappmann; W N Lipscomb; G H Braus
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-05       Impact factor: 11.205

2.  Both chloroplast and mitochondrial NADH dehydrogenase subunit 5 genes are transcribed in pea.

Authors:  J Park; C Breitenberger
Journal:  Biochem Genet       Date:  1992-10       Impact factor: 1.890

3.  Identification of mRNAs associated with programmed cell death in immature thymocytes.

Authors:  G P Owens; W E Hahn; J J Cohen
Journal:  Mol Cell Biol       Date:  1991-08       Impact factor: 4.272

4.  Differential expression of individual genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in Lemna gibba.

Authors:  J Silverthorne; C F Wimpee; T Yamada; S A Rolfe; E M Tobin
Journal:  Plant Mol Biol       Date:  1990-07       Impact factor: 4.076

5.  Aleurones from a Barley with Low [alpha]-Amylase Activity Become Highly Responsive to Gibberellin When Detached from the Starchy Endosperm.

Authors:  R. W. Skadsen
Journal:  Plant Physiol       Date:  1993-05       Impact factor: 8.340

6.  The effects of transcription on the nucleosome structure of four Dictyostelium genes.

Authors:  J Pavlovic; E Banz; R W Parish
Journal:  Nucleic Acids Res       Date:  1989-03-25       Impact factor: 16.971

7.  Maize pyruvate decarboxylase mRNA is induced anaerobically.

Authors:  P M Kelley
Journal:  Plant Mol Biol       Date:  1989-08       Impact factor: 4.076

8.  Specific differentiation events induced by erythropoietin in cells infected in vitro with the anemia strain of Friend virus.

Authors:  M J Koury; M C Bondurant; D T Duncan; S B Krantz; W D Hankins
Journal:  Proc Natl Acad Sci U S A       Date:  1982-01       Impact factor: 11.205

9.  beta-globin transcript found in induced murine erythroleukemia cells is homologous to the beta h0 and beta h1 genes.

Authors:  B A Brown; R W Padgett; S C Hardies; C A Hutchison; M H Edgell
Journal:  Proc Natl Acad Sci U S A       Date:  1982-05       Impact factor: 11.205

10.  The gvpA/C cluster of Anabaena flos-aquae has multiple copies of a gene encoding GvpA.

Authors:  P K Hayes; R S Powell
Journal:  Arch Microbiol       Date:  1995-07       Impact factor: 2.552
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