Literature DB >> 6329678

A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.

C Traboni, G Ciliberto, R Cortese.   

Abstract

We present a novel general method for localized mutagenesis. The DNA segment to be mutagenized is inserted in the beta-galactosidase gene of a M13-lac vector, generally causing loss of beta-galactosidase function by generation of frameshifts or nonsense codons. Mutations in the inserted DNA which restore beta-galactosidase function are readily detected and analyzed. The application of this method to the promoter of an eukaryotic (Caenorhabditis elegans) tRNAPro gene has allowed the isolation of several mutants altered in transcription.

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Year:  1982        PMID： 6329678      PMCID： PMC553061          DOI： 10.1002/j.1460-2075.1982.tb01184.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  35 in total

1.  Base pairing of RNA I with its complementary sequence in the primer precursor inhibits ColE1 replication.

Authors:  R M Lacatena; G Cesareni
Journal:  Nature       Date:  1981-12-17       Impact factor: 49.962

Review 2.  Directed mutagenesis.

Authors:  D Shortle; D DiMaio; D Nathans
Journal:  Annu Rev Genet       Date:  1981       Impact factor: 16.830

3.  Region- and strand-specific mutagensis of a recombinant plasmid.

Authors:  P E Giza; D M Schmit; B L Murr
Journal:  Gene       Date:  1981-12       Impact factor: 3.688

4.  Shotgun DNA sequencing using cloned DNase I-generated fragments.

Authors:  S Anderson
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971

5.  Specific transcription of mouse ribosomal DNA in a cell-free system that mimics control in vivo.

Authors:  I Grummt
Journal:  Proc Natl Acad Sci U S A       Date:  1981-02       Impact factor: 11.205

6.  Site-directed mutagenesis of a tRNA gene: base alterations in the coding region affect transcription.

Authors:  M S Ciampi; D A Melton; R Cortese
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

7.  DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.

Authors:  J L Manley; A Fire; A Cano; P A Sharp; M L Gefter
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205

8.  Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements.

Authors:  G Galli; H Hofstetter; M L Birnstiel
Journal:  Nature       Date:  1981-12-17       Impact factor: 49.962

9.  A split promoter for a eucaryotic tRNA gene.

Authors:  H Hofstetter; A Kressman; M L Birnstiel
Journal:  Cell       Date:  1981-05       Impact factor: 41.582

10.  A system for shotgun DNA sequencing.

Authors:  J Messing; R Crea; P H Seeburg
Journal:  Nucleic Acids Res       Date:  1981-01-24       Impact factor: 16.971
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  34 in total

1.  Quantitative codon optimisation of DNA libraries encoding sub-random peptides: design and characterisation of a novel library encoding transmembrane domain peptides.

Authors:  Ola Larsson; Dorit Thormeyer; Arian Asinger; Björn Wihlén; Claes Wahlestedt; Zicai Liang
Journal:  Nucleic Acids Res       Date:  2002-12-01       Impact factor: 16.971

2.  Neural BC1 RNA: cDNA clones reveal nonrepetitive sequence content.

Authors:  T M DeChiara; J Brosius
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

3.  A tandemly repeated DNA family originated from SINE-related elements in the European plethodontid salamanders (Amphibia, Urodela).

Authors:  R Batistoni; G Pesole; S Marracci; I Nardi
Journal:  J Mol Evol       Date:  1995-06       Impact factor: 2.395

4.  Presence of a limited number of essential nucleotides in the promoter region of mouse ribosomal RNA gene.

Authors:  T Kishimoto; M Nagamine; T Sasaki; N Takakusa; T Miwa; R Kominami; M Muramatsu
Journal:  Nucleic Acids Res       Date:  1985-05-24       Impact factor: 16.971

5.  Point mutations in the 5' ICR and anticodon region of a Drosophila tRNAArg gene decrease in vitro transcription.

Authors:  T S Stewart; D Söll; S Sharp
Journal:  Nucleic Acids Res       Date:  1985-01-25       Impact factor: 16.971

6.  Site specific mutagenesis: insertion of single noncomplementary nucleotides at specified sites by error-directed DNA polymerization.

Authors:  R A Zakour; E A James; L A Loeb
Journal:  Nucleic Acids Res       Date:  1984-08-24       Impact factor: 16.971

7.  The requirement for the A block promoter element in tRNA gene transcription in vitro depends on the ionic environment.

Authors:  O S Gabrielsen; T B Oyen
Journal:  Nucleic Acids Res       Date:  1987-07-24       Impact factor: 16.971

8.  An unusually high number of direct repeats detected by sequence analysis of the dispersed EcoRI-family fragments in Lupinus luteus L.

Authors:  T Sakowicz; G Gałązka; A Konarzewska; M Kwinkowski; J Kłysik
Journal:  Planta       Date:  1986-06       Impact factor: 4.116

9.  Termination of translation in bacteria may be modulated via specific interaction between peptide chain release factor 2 and the last peptidyl-tRNA(Ser/Phe).

Authors:  A L Arkov; S V Korolev; L L Kisselev
Journal:  Nucleic Acids Res       Date:  1993-06-25       Impact factor: 16.971

10.  5' contexts of Escherichia coli and human termination codons are similar.

Authors:  A L Arkov; S V Korolev; L L Kisselev
Journal:  Nucleic Acids Res       Date:  1995-11-25       Impact factor: 16.971
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