Literature DB >> 6328432

Characterization of the genes coding for the Eco RV restriction and modification system of Escherichia coli.

L Bougueleret, M Schwarzstein, A Tsugita, M Zabeau.   

Abstract

A plasmid encoding the recently described Eco RV restriction and modification system has been isolated and characterized. This plasmid, pLB1 , is 6.2 kb long and carries only the Eco RV genes. A subclone of 3 kb has been inserted in pBR322. The relative positions of the endonuclease and the methylase genes were determined by the construction of a set of overlapping deletions generated by Bal31 resection. The DNA sequence of a 2.2 kb fragment containing the two genes was determined. The two genes are transcribed divergently from a 310 bp region and the assignment of the coding region has been confirmed by direct aminoacid sequence analysis. Possible mechanisms of regulation of the endonuclease gene expression at the translational level are proposed and discussed.

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Year:  1984        PMID： 6328432      PMCID： PMC318777          DOI： 10.1093/nar/12.8.3659

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  21 in total

1.  Agarose slab-gel electrophoresis equipment.

Authors:  B Sugden; B De Troy; R J Roberts; J Sambrook
Journal:  Anal Biochem       Date:  1975-09       Impact factor: 3.365

2.  A restriction endonuclease analysis of the bacterial plasmid controlling the ecoRI restriction and modification of DNA.

Authors:  M Betlach; V Hershfield; L Chow; W Brown; H Goodman; H W Boyer
Journal:  Fed Proc       Date:  1976-07

3.  Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

Authors:  M Dagert; S D Ehrlich
Journal:  Gene       Date:  1979-05       Impact factor: 3.688

Review 4.  Structure and function of phage RNA.

Authors:  C Weissmann; M A Billeter; H M Goodman; J Hindley; H Weber
Journal:  Annu Rev Biochem       Date:  1973       Impact factor: 23.643

5.  Codon frequencies in 119 individual genes confirm consistent choices of degenerate bases according to genome type.

Authors:  R Grantham; C Gautier; M Gouy
Journal:  Nucleic Acids Res       Date:  1980-05-10       Impact factor: 16.971

6.  A-protein gene of bacteriophage MS2.

Authors:  W Fiers; R Contreras; F Duerinck; G Haegmean; J Merregaert; W M Jou; A Raeymakers; G Volckaert; M Ysebaert; J Van de Kerckhove; F Nolf; M Van Montagu
Journal:  Nature       Date:  1975-07-24       Impact factor: 49.962

7.  Sequence analysis of the DNA encoding the Eco RI endonuclease and methylase.

Authors:  P J Greene; M Gupta; H W Boyer; W E Brown; J M Rosenberg
Journal:  J Biol Chem       Date:  1981-03-10       Impact factor: 5.157

8.  DNA sequences of structural genes for Eco RI DNA restriction and modification enzymes.

Authors:  A K Newman; R A Rubin; S H Kim; P Modrich
Journal:  J Biol Chem       Date:  1981-03-10       Impact factor: 5.157

9.  Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.

Authors:  J Garnier; D J Osguthorpe; B Robson
Journal:  J Mol Biol       Date:  1978-03-25       Impact factor: 5.469

10.  Molecular cloning of seven mouse immunoglobulin kappa chain messenger ribonucleic acids.

Authors:  N M Gough; E A Webb; S Cory; J M Adams
Journal:  Biochemistry       Date:  1980-06-10       Impact factor: 3.162
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  52 in total

1.  Evidence of horizontal transfer of the EcoO109I restriction-modification gene to Escherichia coli chromosomal DNA.

Authors:  K Kita; J Tsuda; T Kato; K Okamoto; H Yanase; M Tanaka
Journal:  J Bacteriol       Date:  1999-11       Impact factor: 3.490

2.  Cloning and sequence analysis of the genes coding for Eco57I type IV restriction-modification enzymes.

Authors:  A Janulaitis; R Vaisvila; A Timinskas; S Klimasauskas; V Butkus
Journal:  Nucleic Acids Res       Date:  1992-11-25       Impact factor: 16.971

3.  Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors:  M McClelland; M Nelson
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

Review 4.  Organization of restriction-modification systems.

Authors:  G G Wilson
Journal:  Nucleic Acids Res       Date:  1991-05-25       Impact factor: 16.971

5.  Molecular cloning and characterization of the gene encoding the adenine methyltransferase M.CviRI from Chlorella virus XZ-6E.

Authors:  C Stefan; Y N Xia; J L Van Etten
Journal:  Nucleic Acids Res       Date:  1991-01-25       Impact factor: 16.971

6.  Stepwise cloning and molecular characterization of the HgiDI restriction-modification system from Herpetosiphon giganteus Hpa2.

Authors:  A Düsterhöft; D Erdmann; M Kröger
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

7.  Sequence and characterization of pvuIIR, the PvuII endonuclease gene, and of pvuIIC, its regulatory gene.

Authors:  T Tao; R M Blumenthal
Journal:  J Bacteriol       Date:  1992-05       Impact factor: 3.490

8.  Evidence for horizontal transfer of the EcoT38I restriction-modification gene to chromosomal DNA by the P2 phage and diversity of defective P2 prophages in Escherichia coli TH38 strains.

Authors:  Keiko Kita; Hideaki Kawakami; Hiroaki Tanaka
Journal:  J Bacteriol       Date:  2003-04       Impact factor: 3.490

9.  The crystal structure of EcoRV endonuclease and of its complexes with cognate and non-cognate DNA fragments.

Authors:  F K Winkler; D W Banner; C Oefner; D Tsernoglou; R S Brown; S P Heathman; R K Bryan; P D Martin; K Petratos; K S Wilson
Journal:  EMBO J       Date:  1993-05       Impact factor: 11.598

10.  Two-step cloning and expression in Escherichia coli of the DNA restriction-modification system StyLTI of Salmonella typhimurium.

Authors:  O De Backer; C Colson
Journal:  J Bacteriol       Date:  1991-02       Impact factor: 3.490
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