Literature DB >> 6327256

Expression of hepatitis B surface antigen in unselected cell culture transfected with recircularized HBV DNA.

Y Wang, M Schäfer-Ridder, C Stratowa, T K Wong, P H Hofschneider.   

Abstract

Hepatitis B virus (HBV) DNA was isolated from the recombinant plasmid pA01 -HBV and recircularized . Immediately after introduction of this DNA into mouse fibroblasts (NIH 3T3) we observed increasing release of hepatitis B surface antigen (HBsAg) into the culture medium. Later production of HBsAg declined to a lower but constant level. No dominant selective marker and foreign promoter were necessary in this system, which therefore can be used for the study of regulation of HBsAg expression.

Entities:  

Mesh：

Substances：

Year:  1982        PMID： 6327256      PMCID： PMC553191          DOI： 10.1002/j.1460-2075.1982.tb00015.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  13 in total

1.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

2.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

3.  Australia antigen: large-scale purification from human serum and biochemical studies of its proteins.

Authors:  J L Gerin; P V Holland; R H Purcell
Journal:  J Virol       Date:  1971-05       Impact factor: 5.103

4.  Expression of cloned hepatitis B virus DNA in human cell cultures.

Authors:  S Z Hirschman; P Price; E Garfinkel; J Christman; G Acs
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

5.  Transcription of the hepatitis B surface antigen gene in mouse cells transformed with cloned viral DNA.

Authors:  C Pourcel; A Louise; M Gervais; N Chenciner; M F Dubois; P Tiollais
Journal:  J Virol       Date:  1982-04       Impact factor: 5.103

6.  Isolation, characterization, and comparison of recombinant DNAs derived from genomes of human hepatitis B virus and woodchuck hepatitis virus.

Authors:  I W Cummings; J K Browne; W A Salser; G V Tyler; R L Snyder; J M Smolec; J Summers
Journal:  Proc Natl Acad Sci U S A       Date:  1980-04       Impact factor: 11.205

7.  An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA.

Authors:  N D Stow; N M Wilkie
Journal:  J Gen Virol       Date:  1976-12       Impact factor: 3.891

8.  Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli.

Authors:  F Galibert; E Mandart; F Fitoussi; P Tiollais; P Charnay
Journal:  Nature       Date:  1979-10-25       Impact factor: 49.962

9.  Excretion of hepatitis B surface antigen particles from mouse cells transformed with cloned viral DNA.

Authors:  M F Dubois; C Pourcel; S Rousset; C Chany; P Tiollais
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205

10.  Expression of the hepatitis B virus surface antigen gene in cell culture by using a simian virus 40 vector.

Authors:  A M Moriarty; B H Hoyer; J W Shih; J L Gerin; D H Hamer
Journal:  Proc Natl Acad Sci U S A       Date:  1981-04       Impact factor: 11.205
View more
  10 in total

Review 1.  Hepatitis B virus components produced by the human hepatoma cell line PLC/PRF/5: do they indicate virus propagation? Brief review.

Authors:  O Marquardt
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574

2.  Antigenic determinants and functional domains in core antigen and e antigen from hepatitis B virus.

Authors:  J Salfeld; E Pfaff; M Noah; H Schaller
Journal:  J Virol       Date:  1989-02       Impact factor: 5.103

3.  Sensitive non-radioactive dot-blot hybridization using DNA probes labelled with chelate group substituted psoralen and quantitative detection by europium ion fluorescence.

Authors:  A Oser; W K Roth; G Valet
Journal:  Nucleic Acids Res       Date:  1988-02-11       Impact factor: 16.971

4.  Expression of hepatitis B antigens with a simian virus 40 vector.

Authors:  H Will; R Cattaneo; E Pfaff; C Kuhn; M Roggendorf; H Schaller
Journal:  J Virol       Date:  1984-05       Impact factor: 5.103

5.  Hepatitis B virus infection of adult human hepatocytes cultured in the presence of dimethyl sulfoxide.

Authors:  P Gripon; C Diot; N Thézé; I Fourel; O Loreal; C Brechot; C Guguen-Guillouzo
Journal:  J Virol       Date:  1988-11       Impact factor: 5.103

6.  Synthesis of hepatitis B surface antigen in mammalian cells: expression of the entire gene and the coding region.

Authors:  O Laub; L B Rall; M Truett; Y Shaul; D N Standring; P Valenzuela; W J Rutter
Journal:  J Virol       Date:  1983-10       Impact factor: 5.103

7.  Enhanced production of hepatitis B surface antigen in NIH 3T3 mouse fibroblasts by using extrachromosomally replicating bovine papillomavirus vector.

Authors:  Y Wang; C Stratowa; M Schaefer-Ridder; J Doehmer; P H Hofschneider
Journal:  Mol Cell Biol       Date:  1983-06       Impact factor: 4.272

8.  Production of hepatitis B virus in vitro by transient expression of cloned HBV DNA in a hepatoma cell line.

Authors:  C M Chang; K S Jeng; C P Hu; S J Lo; T S Su; L P Ting; C K Chou; S H Han; E Pfaff; J Salfeld
Journal:  EMBO J       Date:  1987-03       Impact factor: 11.598

9.  Co-transcribed 3' host sequences augment expression of integrated hepatitis B virus DNA.

Authors:  A F von Loringhoven; S Koch; P H Hofschneider; R Koshy
Journal:  EMBO J       Date:  1985-01       Impact factor: 11.598

10.  Hepatitis B virus transcription in the infected liver.

Authors:  R Cattaneo; H Will; H Schaller
Journal:  EMBO J       Date:  1984-09       Impact factor: 11.598
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.