Literature DB >> 6326832

Preparation of ligation intermediates and related polynucleotide pyrophosphates.

B C Chu, L E Orgel.   

Abstract

Unprotected oligonucleotides and oligodeoxynucleotides terminated with an unhindered 5'-phosphate group react with nucleoside 5'- phosphorimidazolides in aqueous solution to give 'capped' pyrophosphates in at least 70% yield. If adenosine 5'- phosphorimidazolide is used as a substrate in the reaction, ligase intermediates are obtained as products.

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Year:  1984        PMID： 6326832     DOI： 10.1016/0167-4781(84)90111-8

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  9 in total

1.  The action of a water-soluble carbodiimide on adenosine-5'-polyphosphates.

Authors:  K E Ng; L E Orgel
Journal:  Nucleic Acids Res       Date:  1987-04-24       Impact factor: 16.971

2.  Mineral catalysis of the formation of dimers of 5'-AMP in aqueous solution: the possible role of montmorillonite clays in the prebiotic synthesis of RNA.

Authors:  J P Ferris; G Ertem; V Agarwal
Journal:  Orig Life Evol Biosph       Date:  1989       Impact factor: 1.950

3.  Reaction on alternating GC oligonucleotide templates.

Authors:  K E Ng; L E Orgel
Journal:  J Mol Evol       Date:  1989-08       Impact factor: 2.395

4.  Montmorillonite catalysis of 30-50 mer oligonucleotides: laboratory demonstration of potential steps in the origin of the RNA world.

Authors:  James P Ferris
Journal:  Orig Life Evol Biosph       Date:  2002-08       Impact factor: 1.950

5.  Dimerization in highly concentrated solutions of phosphoimidazolide activated mononucleotides.

Authors:  A Kanavarioti
Journal:  Orig Life Evol Biosph       Date:  1997-08       Impact factor: 1.950

6.  N-methylimidazolium chloride-catalyzed pyrophosphate formation: application to the synthesis of Lipid I and NDP-sugar donors.

Authors:  Hirokazu Tsukamoto; Daniel Kahne
Journal:  Bioorg Med Chem Lett       Date:  2011-04-22       Impact factor: 2.823

7.  Selective cleavage of pyrophosphate linkages.

Authors:  J Visscher; A W Schwartz
Journal:  Nucleic Acids Res       Date:  1992-11-11       Impact factor: 16.971

8.  Information transfer from peptide nucleic acids to RNA by template-directed syntheses.

Authors:  J G Schmidt; P E Nielsen; L E Orgel
Journal:  Nucleic Acids Res       Date:  1997-12-01       Impact factor: 16.971

9.  Oligomerization of activated D- and L-guanosine mononucleotides on templates containing D- and L-deoxycytidylate residues.

Authors:  I A Kozlov; S Pitsch; L E Orgel
Journal:  Proc Natl Acad Sci U S A       Date:  1998-11-10       Impact factor: 11.205
  9 in total

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