| Literature DB >> 6323309 |
M J Macchi, J G Woodward, E McLaughlin-Taylor, J Griffin, L Hood, J A Frelinger.
Abstract
We have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2Dp-specific monoclonal antibodies. The cell line L12a did not react with Kp-specific monoclonal antibodies. This identification was confirmed by mapping a 2.5 kb Bam H1 restriction fragment present in the lambda 12a DNA clone to the D-TL region of H-2p. Only a single 8.8 kb Bam H1 fragment can be assigned to Kp by restriction fragment length polymorphism, while many others map to the D-TL interval. A restriction map of lambda 12a is presented.Entities:
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Year: 1984 PMID: 6323309 DOI: 10.1007/bf00364763
Source DB: PubMed Journal: Immunogenetics ISSN: 0093-7711 Impact factor: 2.846