Literature DB >> 6322118

Osmium tetroxide: a new probe for site-specific distortions in supercoiled DNAs.

G C Glikin, M Vojtískova, L Rena-Descalzi, E Palecek.   

Abstract

Supercoiled plasmids Col E1 and cDm 506 (a Col E1 derivative carrying the D. melanogaster histone gene repeat) were treated with OsO4 in presence of pyridine and the reaction products were analyzed using different approaches. Gel electrophoresis showed that OsO4 binding to supercoiled DNA induced its relaxation without nicking. The amount of osmium bound to DNA (as determined electrochemically) increased with the extent of DNA relaxation. As a result of osmium modification of supercoiled cDm 506, a single denaturation "bubble" was observed in the electron microscope. Mapping of the osmium binding site by S1 nuclease cleavage followed by restriction enzyme digestion has revealed one major site in the intergenic spacer between the H1 and H3 histone genes of D. melanogaster. This site differs from the site cleaved by S1 nuclease in supercoiled DNA in the absence of osmium.

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Year:  1984        PMID： 6322118      PMCID： PMC318611          DOI： 10.1093/nar/12.3.1725

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  21 in total

Review 1.  Structure and reactions of closed duplex DNA.

Authors:  W R Bauer
Journal:  Annu Rev Biophys Bioeng       Date:  1978

2.  The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications.

Authors:  R P Lifton; M L Goldberg; R W Karp; D S Hogness
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1978

3.  Cruciform structures in supercoiled DNA.

Authors:  N Panayotatos; R D Wells
Journal:  Nature       Date:  1981-02-05       Impact factor: 49.962

4.  The inverted repeat as a recognizable structural feature in supercoiled DNA molecules.

Authors:  D M Lilley
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

5.  Molecular microscopy of labeled polynucleotides: stability of osmium atoms.

Authors:  M D Cole; J W Wiggins; M Beer
Journal:  J Mol Biol       Date:  1977-12-05       Impact factor: 5.469

6.  Determination of base sequence in nucleic acids with the electron microscope. V. The thymine-specific reactions of osmium tetroxide with deoxyribonucleic acid and its components.

Authors:  M Beer; S Stern; D Carmalt; K H Mohlhenrich
Journal:  Biochemistry       Date:  1966-07       Impact factor: 3.162

7.  Salt-induced conformational changes of poly(dA-dT).

Authors:  M Vorlícková; J Kypr; V Kleinwächter; E Palecek
Journal:  Nucleic Acids Res       Date:  1980-09-11       Impact factor: 16.971

8.  Osmium-labeled polynucleotides. The reaction of osmium tetroxide with deoxyribonucleic acid and synthetic polynucleotides in the presence of tertiary nitrogen donor ligands.

Authors:  C H Chang; M Beer; L G Marzilli
Journal:  Biochemistry       Date:  1977-01-11       Impact factor: 3.162

9.  Determination of nanogram quantities of osmium-labeled nucleic acids by stripping (inverse) voltammetry.

Authors:  E Palecek; M A Hung
Journal:  Anal Biochem       Date:  1983-07-15       Impact factor: 3.365

10.  Left-handed DNA in restriction fragments and a recombinant plasmid.

Authors:  J Kłysik; S M Stirdivant; J E Larson; P A Hart; R D Wells
Journal:  Nature       Date:  1981-04-23       Impact factor: 49.962
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  6 in total

1.  Detection of thymine [2+2] photodimer repair in DNA: selective reaction of KMnO4.

Authors:  D Ramaiah; T Koch; H Orum; G B Schuster
Journal:  Nucleic Acids Res       Date:  1998-09-01       Impact factor: 16.971

2.  Ozonolysis of supercoiled pBR322 DNA resulting in strand scission to open circular DNA.

Authors:  K Sawadaishi; K Miura; E Ohtsuka; T Ueda; K Ishizaki; N Shinriki
Journal:  Nucleic Acids Res       Date:  1985-10-25       Impact factor: 16.971

3.  (A-T)n tracts embedded in random sequence DNA--formation of a structure which is chemically reactive and torsionally deformable.

Authors:  J A McClellan; E Palecek; D M Lilley
Journal:  Nucleic Acids Res       Date:  1986-12-09       Impact factor: 16.971

4.  Stress-induced cruciform formation in a cloned d(CATG)10 sequence.

Authors:  L H Naylor; D M Lilley; J H van de Sande
Journal:  EMBO J       Date:  1986-09       Impact factor: 11.598

5.  The supercoil-stabilised cruciform of ColE1 is hyper-reactive to osmium tetroxide.

Authors:  D M Lilley; E Palecek
Journal:  EMBO J       Date:  1984-05       Impact factor: 11.598

6.  Location of B- and Z-DNA in the chromosomes of a primitive eukaryote dinoflagellate.

Authors:  M O Soyer-Gobillard; M L Géraud; D Coulaud; M Barray; B Théveny; B Révet; E Delain
Journal:  J Cell Biol       Date:  1990-08       Impact factor: 10.539
  6 in total

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