Literature DB >> 6321157

Isolation of cDNA clones for the human transferrin receptor.

C Schneider, M Kurkinen, M Greaves.   

Abstract

A cDNA clone bank containing 30 000 clones was constructed from sucrose gradient-fractionated mRNA from human placenta. mRNA coding for transferrin receptor (TR) was enriched by polysome immuno-adsorbed chromatography with monospecific rabbit IgG and protein-A Sepharose. The library was screened for hybridisation to 32P-labelled cDNA synthesised from immunoselected TR mRNA and from poly(A)+ RNA of the polysome fraction that failed to bind to protein-A Sepharose. Plasmids isolated from colonies showing hybridisation only to the probe made from immunoselected mRNA were then subjected to hybrid selection. Two clones, pTR-48 and pTR-67, were able to hybridise the mRNA coding for the TR.

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Year:  1983        PMID： 6321157      PMCID： PMC555443          DOI： 10.1002/j.1460-2075.1983.tb01732.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  16 in total

1.  Modulation of cell surface iron transferrin receptors by cellular density and state of activation.

Authors:  J W Larrick; P Cresswell
Journal:  J Supramol Struct       Date:  1979

2.  Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones.

Authors:  S E Hutchings; G H Sato
Journal:  Proc Natl Acad Sci U S A       Date:  1978-02       Impact factor: 11.205

3.  Purification of low-abundance messenger RNAs from rat liver by polysome immunoadsorption.

Authors:  J P Kraus; L E Rosenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

4.  Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library.

Authors:  D M Helfman; J R Feramisco; J C Fiddes; G P Thomas; S H Hughes
Journal:  Proc Natl Acad Sci U S A       Date:  1983-01       Impact factor: 11.205

5.  A rapid boiling method for the preparation of bacterial plasmids.

Authors:  D S Holmes; M Quigley
Journal:  Anal Biochem       Date:  1981-06       Impact factor: 3.365

6.  Structural features of the cell surface receptor for transferrin that is recognized by the monoclonal antibody OKT9.

Authors:  C Schneider; R Sutherland; R Newman; M Greaves
Journal:  J Biol Chem       Date:  1982-07-25       Impact factor: 5.157

7.  Modulation of T leukaemic cell phenotype with phorbol ester.

Authors:  D Delia; M F Greaves; R A Newman; D R Sutherland; J Minowada; P Kung; G Goldstein
Journal:  Int J Cancer       Date:  1982-01-15       Impact factor: 7.396

8.  Plasmid screening at high colony density.

Authors:  D Hanahan; M Meselson
Journal:  Gene       Date:  1980-06       Impact factor: 3.688

9.  Cell-free synthesis and membrane insertion of mouse H-2Dd histocompatibility antigen and beta 2-microglobulin.

Authors:  B Dobberstein; H Garoff; G Warren; P J Robinson
Journal:  Cell       Date:  1979-08       Impact factor: 41.582

10.  Ubiquitous cell-surface glycoprotein on tumor cells is proliferation-associated receptor for transferrin.

Authors:  R Sutherland; D Delia; C Schneider; R Newman; J Kemshead; M Greaves
Journal:  Proc Natl Acad Sci U S A       Date:  1981-07       Impact factor: 11.205
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  8 in total

1.  Identification and characterization of the chicken transferrin receptor.

Authors:  J A Schmidt; J Marshall; M J Hayman
Journal:  Biochem J       Date:  1985-12-15       Impact factor: 3.857

2.  Isolation of cDNA clones derived from a cellular gene transcriptionally induced by herpes simplex virus.

Authors:  R Patel; W L Chan; L M Kemp; N B La Thangue; D S Latchman
Journal:  Nucleic Acids Res       Date:  1986-07-25       Impact factor: 16.971

3.  Deletional analysis of the promoter region of the human transferrin receptor gene.

Authors:  J L Casey; B Di Jeso; K K Rao; T A Rouault; R D Klausner; J B Harford
Journal:  Nucleic Acids Res       Date:  1988-01-25       Impact factor: 16.971

4.  Transcriptional regulation by iron of the gene for the transferrin receptor.

Authors:  K Rao; J B Harford; T Rouault; A McClelland; F H Ruddle; R D Klausner
Journal:  Mol Cell Biol       Date:  1986-01       Impact factor: 4.272

5.  Binding of transferrin to the core protein of fibroblast proteoheparan sulfate.

Authors:  L A Fransson; I Carlstedt; L Cöster; A Malmström
Journal:  Proc Natl Acad Sci U S A       Date:  1984-09       Impact factor: 11.205

6.  Two genetic loci participate in the regulation by iron of the gene for the human transferrin receptor.

Authors:  J L Casey; B Di Jeso; K Rao; R D Klausner; J B Harford
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205

7.  Sex-dependent expression of mouse testosterone 16 alpha-hydroxylase (cytochrome P-450(16) alpha): cDNA cloning and pretranslational regulation.

Authors:  N Harada; M Negishi
Journal:  Proc Natl Acad Sci U S A       Date:  1985-04       Impact factor: 11.205

8.  The transmembrane segment of the human transferrin receptor functions as a signal peptide.

Authors:  M Zerial; P Melancon; C Schneider; H Garoff
Journal:  EMBO J       Date:  1986-07       Impact factor: 11.598
  8 in total

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