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Literature DB >> 273251

Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones.

Abstract

HeLa cells grow in a nutritionally complete synthetic medium (Ham's F12) supplemented with insulin, transferrin, hydrocortisone (aldosterone), fibroblast growth factor, and epidermal growth factor. This hormone-supplemented medium supports clonal growth, long-term cultivation, and a growth rate equal to that of serum-supplemented medium. The omission of any one of the five components results in less than maximal cell growth.

Entities: Chemical Gene

Mesh：

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Year: 1978 PMID： 273251 PMCID： PMC411365 DOI： 10.1073/pnas.75.2.901

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

16 in total

1. CHEMICALLY DEFINED MEDIA FOR CULTIVATION OF LONG-TERM CELL STRAINS FROM FOUR MAMMALIAN SPECIES.

Authors: V J EVANS; J C BRYANT; H A KERR; E L SCHILLING
Journal: Exp Cell Res Date: 1964-12 Impact factor: 3.905

2. CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM.

Authors: R G HAM
Journal: Proc Natl Acad Sci U S A Date: 1965-02 Impact factor: 11.205

3. Growth factors for mammalian cells in culture.

Authors: I LIEBERMAN; P OVE
Journal: J Biol Chem Date: 1959-10 Impact factor: 5.157

4. A hormonally controlled serum factor which stimulates sulfate incorporation by cartilage in vitro.

Authors: W D SALMON; W H DAUGHADAY
Journal: J Lab Clin Med Date: 1957-06

5. Nutrition needs of mammalian cells in tissue culture.

Authors: H EAGLE
Journal: Science Date: 1955-09-16 Impact factor: 47.728

6. A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE: THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORS.

Authors: T T Puck; P I Marcus
Journal: Proc Natl Acad Sci U S A Date: 1955-07-15 Impact factor: 11.205

7. Pituitary extracts and steroid hormones in the control of 3T3 cell growth.

Authors: H A Armelin
Journal: Proc Natl Acad Sci U S A Date: 1973-09 Impact factor: 11.205

8. Long-term cultivation of mammalian cell strains in protein- and lipid-free chemically defined synthetic media.

Authors: T Takaoka; H Katsuta
Journal: Exp Cell Res Date: 1971-08 Impact factor: 3.905

9. Growth response to hormones by a new rat ovary cell line.

Authors: J L Clark; K I Jones; D Gospodarowicz; G H Sato
Journal: Nat New Biol Date: 1972-04-12

10. The specific amino acid requirements of a human carcinoma cell (Stain HeLa) in tissue culture.

Authors: H EAGLE
Journal: J Exp Med Date: 1955-07-01 Impact factor: 14.307

54 in total

1. Serum-free media for murine erythroleukemia cells still not as good as serum-supplemented media.

Authors: G S Ahearn; C C Daehler; S K Majumdar
Journal: In Vitro Cell Dev Biol Date: 1992-04

2. Malignant transformation of human keratinocytes during adaptation to autotrophy.

Authors: M Hill; J Hillova; R Mariage-Samson
Journal: In Vitro Cell Dev Biol Date: 1991-04

3. Cultivation of HeLa cells with fetal bovine serum or Ultroser G: effects on the plasma membrane constitution.

Authors: Y Blixt; A Valeur; E Everitt
Journal: In Vitro Cell Dev Biol Date: 1990-07

4. Identification of transferrin receptors on the surface of human cultured cells.

Authors: T A Hamilton; H G Wada; H H Sussman
Journal: Proc Natl Acad Sci U S A Date: 1979-12 Impact factor: 11.205

5. Suspension culture of separated follicles consisting of differentiated thyroid epithelial cells.

Authors: L Nitsch; S H Wollman
Journal: Proc Natl Acad Sci U S A Date: 1980-01 Impact factor: 11.205

6. A serum-free medium for the growth of muscle cells in culture.

Authors: J R Florini; S B Roberts
Journal: In Vitro Date: 1979-12

7. Comparison of the kinetics of cycling of the transferrin receptor in the presence or absence of bound diferric transferrin.

Authors: N Gironès; R J Davis
Journal: Biochem J Date: 1989-11-15 Impact factor: 3.857

8. Growth effects of epidermal growth factor (EGF) and a monoclonal antibody against the EGF receptor on four glioma cell lines.

Authors: M H Werner; P A Humphrey; D D Bigner; S H Bigner
Journal: Acta Neuropathol Date: 1988 Impact factor: 17.088

9. The effect of desferrioxamine on transferrin receptors, the cell cycle and growth rates of human leukaemic cells.

Authors: A Bomford; J Isaac; S Roberts; A Edwards; S Young; R Williams
Journal: Biochem J Date: 1986-05-15 Impact factor: 3.857

10. Survival of BSC-1 cells through the maintenance of cell volume brought about by epidermal growth factor depends on attachment to the substratum.

Authors: Y Shiba; Y Kanno
Journal: Experientia Date: 1990-05-15