Literature DB >> 6318599

Superoxide dismutase assay using alkaline dimethylsulfoxide as superoxide anion-generating system.

K Hyland, E Voisin, H Banoun, C Auclair.   

Abstract

Alkaline dimethylsulfoxide as a superoxide anion-generating system in association with cytochrome c as a superoxide anion-indicating scavenger has been used to develop a new assay for superoxide dismutase. The assay is sensitive (one unit of enzymatic activity is provided by 110 ng of purified copper-containing superoxide dismutase) and highly specific. The nature of this system prevents the usual interferences and its simplicity allows for multiple, rapid measurements of superoxide dismutase activity in biological preparations using either normal or automated procedures.

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Year:  1983        PMID： 6318599     DOI： 10.1016/0003-2697(83)90684-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  12 in total

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3.  The Superoxide Synthases of Plasma Membrane Preparations from Cultured Rose Cells.

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Journal:  Plant Physiol       Date:  1996-02       Impact factor: 8.340

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Authors:  J Amiel; J C Maziere; I Beucler; M Koenig; L Reutenauer; N Loux; D Bonnefont; C Féo; P Landrieu
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5.  Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress.

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8.  Proteomic analysis of salt-responsive proteins in the leaves of mangrove Kandelia candel during short-term stress.

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9.  Antioxidative and radioprotective potential of rutin and quercetin in Swiss albino mice exposed to gamma radiation.

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10.  Expression of Mn-Superoxide Dismutase Gene in Nontumorigenic and Tumorigenic Human Mammary Epithelial Cells.

Authors:  Sutapa Mukhopadhyay; Salil K Das; Shyamali Mukherjee
Journal:  J Biomed Biotechnol       Date:  2004
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