Literature DB >> 6316322

Targeted selection of recombinant clones through gene dosage effects.

J Rine, W Hansen, E Hardeman, R W Davis.   

Abstract

The availability of multicopy plasmid vectors for the yeast Saccharomyces cerevisiae allows the selective amplification of individual segments of the genome. Increased dosage of particular genes results in overproduction of specific gene products and thereby confers resistance to certain metabolic inhibitors. Advantage was taken of this fact to isolate recombinant clones that increase the activities of the enzymes UDP-N-acetylglucosamine-1-P transferase and 3-hydroxy-3-methylglutaryl-CoA reductase.

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Year:  1983        PMID： 6316322      PMCID： PMC390063          DOI： 10.1073/pnas.80.22.6750

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  31 in total

1.  Competitive inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase by ML-236A and ML-236B fungal metabolites, having hypocholesterolemic activity.

Authors:  A Endo; M Kuroda; K Tanzawa
Journal:  FEBS Lett       Date:  1976-12-31       Impact factor: 4.124

2.  Tunicamycin--an inhibitor of yeast glycoprotein synthesis.

Authors:  S C Kuo; J O Lampen
Journal:  Biochem Biophys Res Commun       Date:  1974-05-07       Impact factor: 3.575

3.  Micro assay for 3-hydroxy-3-methylglutaryl-CoA reductase in rat liver and in L-cell fibroblasts.

Authors:  D J Shapiro; J L Nordstrom; J J Mitschelen; V W Rodwell; R T Schimke
Journal:  Biochim Biophys Acta       Date:  1974-12-29

4.  The biosynthesis of carbamoyl phosphate in Saccharomyces cerevisiae.

Authors:  F Lacroute; A Piérard; M Grenson; J M Wiame
Journal:  J Gen Microbiol       Date:  1965-07

5.  Transposable elements associated with constitutive expression of yeast alcohol dehydrogenase II.

Authors:  V M Williamson; E T Young; M Ciriacy
Journal:  Cell       Date:  1981-02       Impact factor: 41.582

6.  Control of cell type in yeast by the mating type locus. The alpha 1-alpha 2 hypothesis.

Authors:  J Strathern; J Hicks; I Herskowitz
Journal:  J Mol Biol       Date:  1981-04-15       Impact factor: 5.469

7.  Genetic map of Saccharomyces cerevisiae.

Authors:  R K Mortimer; D Schild
Journal:  Microbiol Rev       Date:  1980-12

8.  Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.

Authors:  K A Nasmyth; S I Reed
Journal:  Proc Natl Acad Sci U S A       Date:  1980-04       Impact factor: 11.205

9.  Functional genetic expression of eukaryotic DNA in Escherichia coli.

Authors:  K Struhl; J R Cameron; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1976-05       Impact factor: 11.205

10.  Biosynthesis and characterization of large dolichyl diphosphate-linked oligosaccharides in Saccharomyces cerevisiae.

Authors:  L Lehle; W Tanner
Journal:  Biochim Biophys Acta       Date:  1978-03-01
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  90 in total

1.  Vertebrate and yeast calmodulin, despite significant sequence divergence, are functionally interchangeable.

Authors:  T N Davis; J Thorner
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

Review 2.  Bugs, drugs and chemical genomics.

Authors:  Terry Roemer; Julian Davies; Guri Giaever; Corey Nislow
Journal:  Nat Chem Biol       Date:  2011-12-15       Impact factor: 15.040

Review 3.  Gene overexpression: uses, mechanisms, and interpretation.

Authors:  Gregory Prelich
Journal:  Genetics       Date:  2012-03       Impact factor: 4.562

4.  Target identification using drug affinity responsive target stability (DARTS).

Authors:  Brett Lomenick; Rui Hao; Nao Jonai; Randall M Chin; Mariam Aghajan; Sarah Warburton; Jianing Wang; Raymond P Wu; Fernando Gomez; Joseph A Loo; James A Wohlschlegel; Thomas M Vondriska; Jerry Pelletier; Harvey R Herschman; Jon Clardy; Catherine F Clarke; Jing Huang
Journal:  Proc Natl Acad Sci U S A       Date:  2009-12-07       Impact factor: 11.205

5.  Characterization of multiple transcripts of the hamster dolichol-P-dependent N-acetylglucosamine-1-P transferase suggests functionally complex expression.

Authors:  G T Huang; K Lennon; M A Kukuruzinska
Journal:  Mol Cell Biochem       Date:  1998-04       Impact factor: 3.396

6.  Combining chemical genomics screens in yeast to reveal spectrum of effects of chemical inhibition of sphingolipid biosynthesis.

Authors:  Danielle Kemmer; Lianne M McHardy; Shawn Hoon; Delphine Rebérioux; Guri Giaever; Corey Nislow; Calvin D Roskelley; Michel Roberge
Journal:  BMC Microbiol       Date:  2009-01-14       Impact factor: 3.605

7.  Cloning and characterization of ERG8, an essential gene of Saccharomyces cerevisiae that encodes phosphomevalonate kinase.

Authors:  Y H Tsay; G W Robinson
Journal:  Mol Cell Biol       Date:  1991-02       Impact factor: 4.272

8.  A binary vector for transferring genomic libraries to plants.

Authors:  C Simoens; T Alliotte; R Mendel; A Müller; J Schiemann; M Van Lijsebettens; J Schell; M Van Montagu; D Inzé
Journal:  Nucleic Acids Res       Date:  1986-10-24       Impact factor: 16.971

9.  Ro 09-1470 is a selective inhibitor of P-450 lanosterol C-14 demethylase of fungi.

Authors:  Y Aoki; F Yoshihara; M Kondoh; Y Nakamura; N Nakayama; M Arisawa
Journal:  Antimicrob Agents Chemother       Date:  1993-12       Impact factor: 5.191

10.  Saccharomyces cerevisiae contains two functional genes encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase.

Authors:  M E Basson; M Thorsness; J Rine
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205
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